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首页> 外文期刊>Stem Cell Research & Therapy >Umbilical cord blood-derived mesenchymal stem cells consist of a unique population of progenitors co-expressing mesenchymal stem cell and neuronal markers capable of instantaneous neuronal differentiation
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Umbilical cord blood-derived mesenchymal stem cells consist of a unique population of progenitors co-expressing mesenchymal stem cell and neuronal markers capable of instantaneous neuronal differentiation

机译：脐带血间充质干细胞由共同表达间充质干细胞的独特祖细胞群和能够瞬时分化神经元的神经元标志物组成

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Introduction Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are self-renewing multipotent progenitors with the potential to differentiate into multiple lineages of mesoderm, in addition to generating ectodermal and endodermal lineages by crossing the germline barrier. In the present study we have investigated the ability of UCB-MSCs to generate neurons, since we were able to observe varying degrees of neuronal differentiation from a few batches of UCB-MSCs with very simple neuronal induction protocols whereas other batches required extensive exposure to combination of growth factors in a stepwise protocol. Our hypothesis was therefore that the human UCB-MSCs would contain multiple types of progenitors with varying neurogenic potential and that the ratio of the progenitors with high and low neurogenic potentials varies in different batches of UCB. Methods In total we collected 45 UCB samples, nine of which generated MSCs that were further expanded and characterized using immunofluorescence, fluorescence-activated cell sorting and RT-PCR analysis. The neuronal differentiation potential of the UCB-MSCs was analyzed with exposure to combination of growth factors. Results We could identify two different populations of progenitors within the UCB-MSCs. One population represented progenitors with innate neurogenic potential that initially express pluripotent stem cell markers such as Oct4, Nanog, Sox2, ABCG2 and neuro-ectodermal marker nestin and are capable of expanding and differentiating into neurons with exposure to simple neuronal induction conditions. The remaining population of cells, typically expressing MSC markers, requires extensive exposure to a combination of growth factors to transdifferentiate into neurons. Interesting to note was that both of these cell populations were positive for CD29 and CD105, indicating their MSC lineage, but showed prominent difference in their neurogenic potential. Conclusion Our results suggest that the expanded UCB-derived MSCs harbor a small unique population of cells that express pluripotent stem cell markers along with MSC markers and possess an inherent neurogenic potential. These pluripotent progenitors later generate cells expressing neural progenitor markers and are responsible for the instantaneous neuronal differentiation; the ratio of these pluripotent marker expressing cells in a batch determines the innate neurogenic potential.
机译：简介脐带血（UCB）来源的间充质干细胞（MSCs）是自我更新的多能祖细胞，除了通过种系壁垒产生外胚层和内胚层谱系外，还可以分化为中胚层的多个谱系。在本研究中，我们已经研究了UCB-MSC产生神经元的能力，因为我们能够通过非常简单的神经元诱导方案从几批UCB-MSC中观察到不同程度的神经元分化，而其他批次则需要大量接触逐步协议中选择生长因子。因此，我们的假设是，人UCB-MSC将包含多种类型的具有不同神经原性潜能的祖细胞，并且具有高和低神经原性潜能的祖细胞的比例在不同批次的UCB中会有所不同。方法我们总共收集了45个UCB样品，其中9个产生了MSC，并通过免疫荧光，荧光激活细胞分选和RT-PCR分析对其进行了进一步的鉴定。通过暴露于生长因子的组合来分析UCB-MSC的神经元分化潜能。结果我们可以在UCB-MSCs中鉴定出两个不同的祖细胞群。一个群体代表具有先天神经源性潜能的祖细胞，这些祖细胞最初表达多能干细胞标记物，例如Oct4，Nanog，Sox2，ABCG2和神经外胚层标记物Nestin，并且能够通过暴露于简单的神经元诱导条件而扩展并分化为神经元。剩下的通常表达MSC标志物的细胞群需要大量接触生长因子的组合才能转分化为神经元。有趣的是，这两个细胞群均对CD29和CD105呈阳性，表明它们具有MSC谱系，但在其神经源性潜力方面表现出显着差异。结论我们的结果表明，扩增的UCB衍生的MSC包含一小群独特的细胞，这些细胞表达多能干细胞标记以及MSC标记，并具有固有的神经源性潜力。这些多能祖细胞随后生成表达神经祖细胞标记的细胞，并负责瞬时神经元分化。这些多能标记表达细胞的比例决定了先天神经源性潜力。

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