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首页> 外文期刊>SLAS Technology >Automation of Human IgG, Glucose, Lactate, and Oxygen Assays on Biomek NXP Span-8 Automation Workstation and PARADIGM Detection Platform
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Automation of Human IgG, Glucose, Lactate, and Oxygen Assays on Biomek NXP Span-8 Automation Workstation and PARADIGM Detection Platform

机译：Biomek NX P Span-8自动化工作站和PARADIGM检测平台上的人IgG，葡萄糖，乳酸和氧气测定的自动化

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When optimizing cell culture media for therapeutic antibody production cell lines, it is very important to monitor parameters such as IgG concentration, glucose and lactate levels, and oxygen consumption in culture medium in an automated high-throughput manner to ensure the desirable growth conditions. In this report, assays for measuring human IgG, glucose, and lactate concentrations, and oxygen consumption were automated using the Biomek NX~(P) Span-8 workstation, and analyzed on the PARADIGM Detection Platform. The human-IgG homogeneous time-resolved fluorescence (HTRF) (All trademarks are the property of their respective owners.) assay from Cisbio Inc. (Cisbio US, Bedford, MA) was automated in a 384-well plate format and detected on the PARADIGM with a HTRF Detection Cartridge. Glucose and lactate assays were automated in a 96-well plate format and detected on the PARADIGM with a Fluorescence Intensity Cartridge. Oxygen consumption was determined by the 96-well calibration-free OxoPlate (All trademarks are the property of their respective owners.) (PreSens Inc., PreSens - Precision Sensing GmbH, Regensburg, Germany) and detected by the PARADIGM with a MultiMode Detection Cartridge.
机译：当优化用于治疗性抗体产生细胞系的细胞培养基时，以自动化的高通量方式监测诸如IgG浓度，葡萄糖和乳酸水平以及培养基中的氧消耗等参数以确保理想的生长条件非常重要。在本报告中，使用Biomek NX〜（P）Span-8工作站自动化了用于测定人IgG，葡萄糖和乳酸浓度以及耗氧量的测定，并在PARADIGM检测平台上进行了分析。来自Cisbio Inc.（Cisbio US，Bedford，MA）的人IgG均相时间分辨荧光（HTRF）（所有商标均为其各自所有者的财产）测定以384孔板形式自动进行，并在带有HTRF检测盒的PARADIGM。葡萄糖和乳酸测定以96孔板形式自动进行，并使用荧光强度盒在PARADIGM上进行检测。耗氧量由96孔免校准OxoPlate（所有商标均为其各自所有者的财产）确定（PreSens Inc.，PreSens-Precision Sensing GmbH，德国雷根斯堡），并由PARADIGM使用多模式检测盒进行检测。 。

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