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首页> 外文期刊>Pain research & management: the journal of the Canadian Pain Society = journal de la socie?te? canadienne pour le traitement de la douleur >Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury
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Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury

机译：姜黄素保护人脐静脉内皮细胞免受H2O2诱导的细胞损伤

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Migraine is a prevalent neurological disorder which causes a huge economic burden on society. It is thought to be a neurovascular disease with oxidative stress might be involved. Curcumin, one of the major ingredients of turmeric, has potent antioxidative and anti-inflammatory properties, but whether it could be used as a potential treatment for migraine remains to be explored. In the present study, human umbilical vein endothelial cells (HUVECs) were pretreated with various concentrations of curcumin (0?μM, 10?μM, 20?μM, 30?μM, 40?μM, and 50?μM) for 12?h, thereby exposed to H2O2 (100?μM) for another 12?h. The viability of HUVECs was tested by the CCK-8 assay, and the activities of antioxidant enzymes including superoxide dismutase (SOD) and glutathione (GSH) were also examined. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were assayed to determine H2O2-induced oxidative stress. In addition, several cell death-related genes (p53, p21, Bax, and Bcl-2) were detected by PCR, and an apoptosis-related protein (caspase3) was evaluated by western blotting. Our results showed that curcumin improved the H2O2-induced decrease of cell viability and antioxidative enzyme activities and decreased the level of oxidative stress. As a conclusion, curcumin could mitigate H2O2-induced oxidative stress and cell death in HUVECs and may be a potential therapeutic drug for migraine.
机译：偏头痛是一种普遍的神经系统疾病，对社会造成巨大的经济负担。被认为是可能与氧化应激有关的神经血管疾病。姜黄素是姜黄的主要成分之一，具有有效的抗氧化和消炎作用，但是否可以用作偏头痛的潜在治疗方法尚待探讨。在本研究中，使用各种浓度的姜黄素（0？μM，10？μM，20？μM，30？μM，40？μM和50？μM）预处理人脐静脉内皮细胞（HUVEC）12？h ，然后再暴露于H2O2（100？μM）中12？h。 HUVEC的生存能力通过CCK-8分析进行了测试，还检测了抗氧化剂酶的活性，包括超氧化物歧化酶（SOD）和谷胱甘肽（GSH）。分析细胞内活性氧（ROS）和丙二醛（MDA），以确定H2O2诱导的氧化应激。另外，通过PCR检测了几种细胞死亡相关基因（p53，p21，Bax和Bcl-2），并通过蛋白质印迹法评估了凋亡相关蛋白（caspase3）。我们的结果表明，姜黄素改善了H2O2诱导的细胞活力和抗氧化酶活性的降低，并降低了氧化应激的水平。结论是，姜黄素可减轻HUVEC中H2O2引起的氧化应激和细胞死亡，并且可能是偏头痛的潜在治疗药物。

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