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首页> 外文期刊>Pakistan Journal of Biological Sciences >Effectiveness of Metarhizium anisopliae and Entomopathogenic Nematodes to Control Oryctes rhinoceros Larvae in the Rainy Season
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Effectiveness of Metarhizium anisopliae and Entomopathogenic Nematodes to Control Oryctes rhinoceros Larvae in the Rainy Season

机译：梅雨季线虫和昆虫致病性线虫在雨季控制稻孔犀幼虫的有效性

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Background and Objective: Metarhizium anisopliae (MET) and entomopathogenic nematodes (EPN) are microorganisms that attack the larvae of Oryctes rhinoceros. The effects of MET, EPN and the combination of both on the O. rhinoceros larvae were studied during the rainy season in Jepara Indonesia. This study aimed to determine the effectiveness of Metarhizium anisopliae and entomopathogenic nematodes to control Oryctes rhinoceros larvae in the rainy season. Materials and Methods: There were four level doses of MET, four level doses of EPN and four mixture of MET and EPN. The experiment used 72 containers that were placed in the garden with coconut palm shade. Five kilograms of organic soil that was mixed with biological control agents (MET, EPN and MET+EPN) and ten O. rhinoceros larvae 3rd instar were put in each other container. The data were analyzed by descriptive analysis. Results: Every larvae mortality was observed once a week and observations are for 8 weeks. The result showed that the larval mortality due to MET treatment occurred on 2nd-7th week. Meanwhile, the larval mortality due to EPN treatment took place on 2nd-8th weeks and the larval mortality due to MET+EPN treatment occurred on 1st-5th weeks. Conclusion: The combination of MET and EPN was simultaneously effective to control O. rhinoceros larvae than separate use of MET or EPN. Result of this study showed that using two agents of biocontrol was more effective, so that it can be beneficial for controlling O. rhinoceros larvae in the field.
机译：背景与目的：介僵线虫（METRhizium anisopliae，METN）和昆虫病原线虫（EPN）是攻击犀牛幼虫的微生物。在雨季期间，在印度尼西亚Jepara考察了MET，EPN以及二者的组合对犀牛幼虫的影响。这项研究的目的是确定在雨季，金属异形体和昆虫致病性线虫防治稻蚜的有效性。材料和方法：有四级剂量的MET，四级剂量的EPN和四级MET和EPN的混合物。实验使用了72个放在椰子树荫下的花园中的容器。将五公斤与生物防治剂（MET，EPN和MET + EPN）混合的有机土壤和十只O. Rhinoceros幼虫第三龄幼虫放入另一个容器中。通过描述性分析对数据进行分析。结果：每个幼虫的死亡率每周观察一次，观察持续8周。结果表明，MET处理引起的幼虫死亡率发生在第2-7周。同时，EPN处理引起的幼虫死亡率在第2-8周发生，而MET + EPN处理引起的幼虫死亡率在1-5周发生。结论：与单独使用MET或EPN相比，MET和EPN的组合同时可有效控制犀牛幼虫。研究结果表明，使用两种生物防治剂更为有效，因此在野外防治犀牛幼体可能是有益的。

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