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首页> 外文期刊>Pakistan journal of botany >Alginate production by a mutant strain of Azotobacter vinelandii using shake flask fermentation
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Alginate production by a mutant strain of Azotobacter vinelandii using shake flask fermentation

机译：使用摇瓶发酵法由葡萄固氮菌的突变菌株生产藻酸盐

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摘要

The present study describes the improvement of parent strain of Azotobacter vinelandii NRRL-14641 for enhanced alginate production using shake flask fermentation. Results indicated that a mutant A. vinelandii EMS-45 obtained after EMS treatment gave 1.55 fold better alginate productions than that of the parent strain. Different cultural conditions were employed to optimize the polymer production in the fermentation broth. It was noted that maximum alginate production (6.17 g/l) was obtained with nitrogen rich phosphate limited medium (NRP) after 110 h of incubation period. The incubation temperature 30°C, pH 7.0, agitation intensity 200 rpm, carbon and nitrogen source were also optimized and sucrose and peptone were found as the best to support 1.55 fold higher alginate than that in un-optimized medium.
机译：本研究描述了摇瓶发酵提高藻酸盐生产的葡萄固氮菌NRRL-14641亲本菌株的改良。结果表明，在EMS处理后获得的突变体A.vinelandii EMS-45产生的藻酸盐产量比亲本菌株高1.55倍。采用不同的培养条件以优化发酵液中的聚合物产量。注意到在孵育110小时后，使用富氮磷酸盐限制培养基（NRP）获得了最大藻酸盐产量（6.17 g / l）。还优化了温育温度30°C，pH 7.0，搅拌强度200 rpm，碳和氮源，发现蔗糖和蛋白ept比未优化培养基中的藻酸盐支持率高1.55倍。

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