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首页> 外文期刊>Pakistan journal of botany >Molecular and epidemiological analysis of multidrug-resistant salmonella paratyphi a obtained from different regions of Pakistan
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Molecular and epidemiological analysis of multidrug-resistant salmonella paratyphi a obtained from different regions of Pakistan

机译：从巴基斯坦不同地区获得的耐多药副伤寒沙门氏菌的分子和流行病学分析

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Incidence of paratyphoid infections caused by Salmonella paratyphi A is significantly mounting high in the country. Indiscriminate use of antibiotics which often results in the development of drug-resistant strains, is making the treatment difficult. This situation becomes worst when organism acquire multidrug resistance (MDR). In the present study, various isolates of MDR S. paratyphi A, obtained from different regions of Pakistan, were analyzed for plasmid profile and multiplex PCR to determine the resistance gene associated with plasmid and chromosomal DNA of S. paratyphi A. Multi-drug resistant isolates of S. paratyphi A obtained during the year 2001-2006 from different regions of Pakistan have been included in this study. These isolates showed resistance to chloramphenical (30μg), ampicillin (10μg), trimethoprim-sulfamethoxazole (23.5μg), and tetracycline (30μg). Some strains were also found resistant to ofloxacin and nalidixic acid. The plasmids from bacterial strains were found to be approximately 220 kbp, harboring antibiotic resistance genes. A multiplex PCR was used to identify any variation in antibiotic resistance genes encoding the MDR phenotypes in clinical isolates of S. paratyphi A. A homogenous pattern of multiplex PCR product revealed that MDR isolates of S. paratyphi A harbored the same resistance genes. This study will help taking effective measures for controlling disease in the region, and the data could be used for future medical reference.
机译：在该国，由副伤寒沙门氏菌A引起的副伤寒感染的发生率显着上升。滥用抗生素通常会导致产生耐药菌株，这使治疗变得困难。当生物获得多药耐药性（MDR）时，这种情况变得最糟。在本研究中，分析了来自巴基斯坦不同地区的多种MDR副伤寒沙门氏菌的分离株的质粒图谱和多重PCR，以确定与副伤寒沙门氏菌的质粒和染色体DNA相关的抗性基因。这项研究包括2001-2006年间从巴基斯坦不同地区获得的副伤寒沙门氏菌A分离株。这些分离株显示出对氯霉素（30μg），氨苄青霉素（10μg），甲氧苄啶-磺胺甲恶唑（23.5μg）和四环素（30μg）的抗性。还发现一些菌株对氧氟沙星和萘啶酸具有抗性。发现来自细菌菌株的质粒约为220kbp，具有抗生素抗性基因。多重PCR用于鉴定副伤寒沙门氏菌临床分离株中编码MDR表型的抗生素抗性基因的任何变异。多重PCR产物的均质模式显示副伤寒沙门氏菌的MDR分离株具有相同的抗性基因。这项研究将有助于采取有效措施来控制该地区的疾病，这些数据可用于将来的医学参考。

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