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首页> 外文期刊>Pakistan journal of botany >Studies on its sequences and systematic classification of Osmanthus
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Studies on its sequences and systematic classification of Osmanthus

机译：桂花序列及其系统分类研究

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In this article, ITS sequences of 20 species in Osmanthus were cloned and researched. It was found that length of ITS sequences ranged from 614bp to 619bp, in which ITS-1 is obviously longer than ITS-2, 5.8S of 20 species is composed of 163bp, and the percentage of G+C in ITS sequence of 20 species is 54.24%-64.56%. Alignment between ITS sequences from 20 species of Osmanthus was performed, and discovered that the similarity coefficient between 20 species is 86.1%-99.7%, the minimum exists between O.suavi and O.attenuatus, the similarity coefficient between O.urceolatus and O.cooperi is the maximum. Furthermore, there are 198 mutation sites and 97 information sites in ITS sequences, respectively 31.53% and 15.45%, and the content of information sites in ITS-2 is more than that in ITS-1. In addition, three MP trees were respectively constructed based on ITS sequences, ITS-1 sequences and ITS-2 sequences, 20 species of Osmanthus were divided into three groups, O.americanus belonged to the first group, O.yunnanensis and O.attenuatus were clustered one group, and the other 17 species belonged to the third group, which is partly consistent with the viewpoint of traditional classification about Osmanthus.
机译：本文对桂花的20种ITS序列进行了克隆和研究。发现ITS序列的长度在614bp至619bp之间，其中ITS-1明显长于ITS-2，其中20种5.8S由163bp组成，G + C在20种ITS中的百分比是54.24％-64.56％。进行了20种桂花ITS序列的比对，发现20种桂花的相似系数为86.1％-99.7％，O.suavi和O.衰减菌之间的最小相似度，O.urceolatus和O.衰减虫之间的相似系数最小。合作社是最大的。 ITS序列中共有198个突变位点和97个信息位点，分别为31.53％和15.45％，ITS-2中信息位点的含量高于ITS-1。此外，根据ITS序列，ITS-1序列和ITS-2序列分别构建了3个MP树，将20种桂花分为三类，美洲O属第一类，云南臭and和弱毒attenuat。桂花被归为一类，其他17种属于第三类，这在一定程度上与传统的桂花分类观点相吻合。

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