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首页> 外文期刊>Pakistan journal of botany >Induced systemic resistance in chickpea against Ascochyta blight by safe chemicals
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Induced systemic resistance in chickpea against Ascochyta blight by safe chemicals

机译：安全化学物质诱导鹰嘴豆对枯萎病的全身抗性

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Chickpea variety, C727 susceptible to Ascochyta rabiei, was grown in small plots of 20 sq feet area. Induction treatments were given by spraying aqueous solutions of 1.0 mM Salicylic acid, 50 mM K2HPO4 (analytical and commercial grade), 0.4 mM Bion and two types of neem leaves extract (50mg/ml, leaves boiled in methanol, methanol was evaporated then residue dissolved in water and in other extract neem leaves were boiled in water) at flowering stage of the plants in triplicates. Control plants were treated with water only. One week after induction treatments, all the plants were challenged with spore suspension of A. rabiei (106spores/ml). Disease data was recorded after disease was fully developed on control plants. Plant tissues were collected at 0, 24, 48 and 72 hours after induction and challenge treatments for biochemical analyses of proteins and peroxidase enzyme activity as the part of defense mechanism. Blight disease was significantly reduced in chickpea variety C727 after spraying the plants with all the chemicals tested. Maximum reduction in the disease was obtained with Salicylic acid, followed by Bion. Slight increase in yield was observed in Bion, K2HPO4(commercial) and neem extract treated plants as compared to control plants but the difference was non-significant. Plant tissues collected at different time intervals from induced and control plants after induction and challenged treatments were subjected to biochemical analyses of total proteins, SDS-PAGE, activity of peroxidase enzyme and Native PAGE for isozymes of peroxidase. A significant increase in total proteins and peroxidase activity was observed after induction treatments with all the chemicals tested. However, electrophoresis indicated that pattern of proteins and isoforms of peroxidase were similar in induced and control plants.
机译：鹰嘴豆品种C727易受狂犬病菌侵染，在面积为20平方英尺的小块土地上种植。通过喷洒1.0 mM水杨酸，50 mM K2HPO4（分析和商业级），0.4 mM Bion水溶液和两种印ne叶提取物（50mg / ml，叶在甲醇中煮沸，将甲醇蒸发，然后将残留物溶解）进行诱导处理。在水和其他提取物中，在植物的开花期将印度ne叶在水中煮沸，一式三份。对照植物仅用水处理。诱导处理后一周，所有植物用狂犬曲霉的孢子悬浮液（106孢子/ ml）攻击。病害在对照植物上完全发育后记录病害数据。诱导和激发处理后0、24、48和72小时收集植物组织，作为防御机制的一部分，对蛋白质和过氧化物酶活性进行生化分析。用所有测试的化学物质喷洒植物后，鹰嘴豆品种C727的枯萎病显着减少。使用水杨酸，然后使用Bion，可以最大程度地减少疾病。在Bion，K2HPO4（商业）和印em提取物处理的植物中，与对照植物相比，产量略有增加，但差异不显着。在诱导和激发处理后，以不同时间间隔从诱导植物和对照植物中收集的植物组织进行总蛋白，SDS-PAGE，过氧化物酶活性和Native PAGE的过氧化物同工酶的生化分析。用所有测试的化学品进行诱导处理后，总蛋白质和过氧化物酶活性均显着增加。然而，电泳表明诱导植物和对照植物中蛋白质和过氧化物酶同工型的模式相似。

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