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首页> 外文期刊>Pakistan journal of botany >Evaluation of ISSR markers to assess genetic variability and relationship among winter triticale (x triticosecale Wittmack) cultivars
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Evaluation of ISSR markers to assess genetic variability and relationship among winter triticale (x triticosecale Wittmack) cultivars

机译：利用ISSR标记评估冬季小黑麦（x triticosecale Wittmack）品种之间的遗传变异和相关性

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The ISSR technique was used to identify genetic relationships in 11 winter hexaploid triticale cultivars "Lasko, Stan-1, Malno, Purdy, AN-34, Tatlicak-97, Karma-2000, Presto, Melez-2001, Mikham-2002, Samur Sorti". Twenty ISSR primers were tested and twelve of them amplified clear and reproducible bands. The number of ISSR fragments generated per primer set ranged from 5 to 31 with fragment sizes varying from 320 to 2700bp. A total of 209 ISSR fragments were detected, of which 159 were polymorphic (76.07%). All cultivars were clearly differentiated by their ISSR fingerprints. Based on UPGMA analysis a dendrogram was constructed and 11 triticale cultivars were grouped in two clusters. Cluster I was the largest, comprising 10 cultivars which can be divided into four subclusters. Only one cultivar, "Stan-1" was positioned in Cluster II. The polymorphic patterns generated by ISSR profiles showed different degrees of genetic relationship among the cultivars studied. Similarity values between cultivars ranged from 0.59 to 0.89. The results indicate that ISSRs may constitute a relatively simple and efficient method for analysing genetic variation in triticale.
机译：ISSR技术用于鉴定11个冬季六倍体小黑麦品种“ Lasko，Stan-1，Malno，Purdy，AN-34，Tatlicak-97，Karma-2000，Presto，Melez-2001，Mikham-2002，Samur Sorti”的遗传关系”。测试了20条ISSR引物，其中12条扩增了清晰且可再现的条带。每个引物组产生的ISSR片段的数目为5至31，片段大小为320至2700bp。共检测到209个ISSR片段，其中159个为多态性（76.07％）。所有品种的ISSR指纹图谱都清楚地区分了它们。根据UPGMA分析，构建了树状图，并将11个小黑麦品种分为两个类。第一类最大，包括10个品种，可分为四个子类。仅一个品种“ Stan-1”位于第二类中。 ISSR图谱产生的多态性模式显示了所研究品种之间不同程度的遗传关系。品种之间的相似度范围为0.59至0.89。结果表明，ISSRs可能是分析黑小麦遗传变异的一种相对简单有效的方法。

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