Crystallization of fructose 1,6-bisphosphatase from the hyperthermophilic Archaeon Thermococcus kodakaraensis

Naeem Rashid; Masood Ahmed Siddiqui; Muhammad Saleem Haider; Muhammad Arshad Javed

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Crystallization of fructose 1,6-bisphosphatase from the hyperthermophilic Archaeon Thermococcus kodakaraensis

机译：从超嗜热古生嗜热球菌中提取果糖1,6-双磷酸酶

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The enzyme Fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11) is one of the key enzymes of the gluconeogenic pathway. It hydrolyses fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate (Pilkis & Claus, 1991). Here we report the crystallization of FBPase from Thermococcus kodakaraensis. This FBPase consists of 375 amino acids, with a molecular weight of 42kDa, and was prepared using an Escherichia coli expression system. The purified recombinant FBPase was crystallised using the sitting drop vapour-diffusion method at 17°C. Crystals grew tetragonally and measured approximately 0.4 mm in the longest dimension.

机译：果糖-1,6-双磷酸酶（FBPase; EC 3.1.3.11）是糖异生途径的关键酶之一。它将1,6-二磷酸果糖水解为6-磷酸果糖和无机磷酸盐（Pilkis＆Claus，1991）。在这里，我们报告了来自嗜热球菌的FBPase的结晶。该FBPase由375个氨基酸组成，分子量为42kDa，并使用大肠杆菌表达系统制备。纯化的重组FBPase在17°C下使用坐滴蒸汽扩散法结晶。晶体呈四边形生长，最长尺寸约为0.4毫米。

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《Pakistan journal of botany》 |2010年第4期|共4页
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Naeem Rashid; Masood Ahmed Siddiqui; Muhammad Saleem Haider; Muhammad Arshad Javed;
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1. Gene cloning and characterization of fructose-1,6-bisphosphate aldolase from the hyperthermophilic archaeon thermococcus kodakaraensis KOD1 [J] . Hiroyuki Imanaka, Toshiaki Fukui, Haruyuki Atomi, Journal of Bioscience and Bioengineering . 2002,第3期

机译：嗜热古生热球菌kodkaraensis KOD1中1,6-双磷酸果糖-1,6-二磷酸果糖酶的基因克隆与鉴定
2. Genetic Evidence Identifying the True Gluconeogenic Fructose-1,6-Bisphosphatase in Thermococcus kodakaraensis and Other Hyperthermophiles [J] . Takaaki Sato, Hiroyuki Imanaka, Naeem Rashid, Journal of bacteriology . 2004,第17期

机译：遗传证据，确定在真热球菌和其他超嗜热菌中真正的糖原性果糖-1,6-双磷酸酶。
3. Crystal structure of a phosphoribosyl anthranilate isomerase from the hyperthermophilic archaeon Thermococcus kodakaraensis [J] . Perveen Sumera, Rashid Naeem, Papageorgiou Anastassios C. Acta crystallographica. Section F, Structural biology communications . 2016,第11期

机译：来自高疗性Archaeon Thermococcus Kodakaraensis的磷酸亚氨基氨基酸酯异构酶的晶体结构
4. Complete genome analysis and development of gene disruption technology in the hyperthermophilic archaeon, Thermococcus kodakaraensis [C] . Imanaka, T., Fukui, . 2005

机译：嗜热古菌柯达热球菌的完整基因组分析和基因破坏技术的发展
5. Biochemistry of the cytosolic isozymes of fructose-1,6-bisphosphatase and fructose-1,6-bisphosphate aldolase from germinated castor oil seeds. [D] . Hodgson, Richard John. 1998

机译：来自发芽的蓖麻油种子中的果糖-1,6-双磷酸酶和果糖-1,6-双磷酸醛缩酶的胞质同工酶的生物化学。
6. Crystallization and preliminary X-ray diffraction analysis of l-threonine dehydrogenase (TDH) from the hyperthermophilic archaeon Thermococcus kodakaraensis [O] . A. Bowyer, H. Mikolajek, J. N. Wright, 2008

机译：嗜热古细菌嗜热球菌l-苏氨酸脱氢酶（TDH）的结晶和初步X射线衍射分析
7. Genetic evidence identifying the true gluconeogenic fructose-1,6-bisphosphatase in Thermococcus kodakaraensis and other hyerthermophiles. [O] . Sato, T, Imanaka, H, Rashid, N, 2004

机译：遗传证据确定了嗜热球菌和其他嗜热菌中真正的糖异生果糖-1,6-双磷酸酶。

Crystallization of fructose 1,6-bisphosphatase from the hyperthermophilic Archaeon Thermococcus kodakaraensis

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