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首页> 外文期刊>Pakistan journal of medical sciences. >Use of supravital toluidine blue staining to improve the efficiency of fine-needle aspiration cytology reportingin comparison to papanicolaou stain
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Use of supravital toluidine blue staining to improve the efficiency of fine-needle aspiration cytology reportingin comparison to papanicolaou stain

机译：与帕帕尼可拉乌氏染色相比，使用上甲苯甲蓝染色提高细针穿刺细胞学报告的效率

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Objective: To see the efficiency, adequacy and accuracy of toluidine blue stained smears of FNAC of Breast thyroid and salivary glands swelling with comparison to conventional stained FNAC smears with Papanicolaou.Methods: A total of 114 aspirates from various sites were included in the study. The smears were stained with toluidine blue and conventional Papanicolaou stain and the cytomorphology of both the smears were compared. The values were tabulated and statistical tests of significance was applied.Results: Of the 114 aspirates included in our study the diagnostic accuracy by using papanicolaou was 78%, while it was upto 100% with supravital toluidine blue stained smears. The percentage of inadequacy was reduced to just 25%. The observations were statistically significant. Breast 37/48 (77%) and Salivary glands 11/48 (23%) respectively. The most commonly used categorization of a five-tier system was used for reporting of breast cytology, with categories ranging from insufficient materials (C1), benign (C2), atypical (C3), suspicious of malignancy (C4), or (C5) frankly malignant. Most of breast lesions were benign 25 (67.56%). There were only 9 (24.32%) malignant cases followed by 2 cases of C-4 and one case of C-3. Benign thyroid lesion were more frequent comprising of 51 (72.27%) cases. One case (1.5%) of papillary carcinoma was found while 13 case were follicular lesions. There were 4 (36.4%) cases of pleomorphic adenoma and 3 (27.3) cases of non-specific sialadenitis. There was one case (9%) of each lesion for mucoepidermoid carcinoma, adenoidcytic carcinoma and benign cyst.Conclusion: Toluidine blue stained study of FNAC improves the diagnostic accuracy by minimizing the smearing and drying artifact, loss of cell sample during fixation and staining which influences the diagnostic accuracy.doi: http://dx.doi.org/10.12669/pjms.315.8411How to cite this:Saba K, Niazi S, Bukhari MH, Imam SF. Use of supravital toluidine blue staining to improve the efficiency of fine-needle aspiration cytology reporting in comparison to papanicolaou stain. Pak J Med Sci 2015;31(5):1146-1151. doi: http://dx.doi.org/10.12669/pjms.315.8411This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
机译：目的：与常规帕潘尼古拉FNAC涂片相比，甲状旁腺甲胺蓝涂片对甲状腺和唾液腺肿胀的FNAC涂片的有效性，充分性和准确性。方法：本研究共包括来自不同部位的114例吸出物。用甲苯胺蓝和常规巴氏染色对涂片进行染色，比较两种涂片的细胞形态。结果列于表中，并进行统计学检验。结果：在我们研究的114例抽吸物中，使用帕帕尼可乐诊断的准确性为78％，而经甲上甲酰甲苯胺蓝涂片的诊断准确率高达100％。不足的比例降低到只有25％。观察结果具有统计学意义。乳房分别为37/48（77％）和唾液腺11/48（23％）。五层系统最常用的分类用于报告乳腺癌细胞学，其分类范围从材料不足（C1），良性（C2），非典型（C3），可疑恶性（C4）或（C5）坦白说是恶性的。大部分乳腺病变为良性25（67.56％）。只有9例（24.32％）恶性肿瘤，其次是2例C-4和1例C-3。甲状腺良性病变更为常见，占51例（72.27％）。发现乳头状癌1例（1.5％），滤泡性病变13例。多形性腺瘤4例（36.4％），非特异性涎腺炎3例（27.3）。粘液表皮样癌，腺样囊性癌和良性囊肿每个病变只有1例（9％）。结论：FNAC的甲苯胺蓝染色研究可通过最大程度地减少涂抹和干燥伪影，固定和染色过程中细胞样品的丢失来提高诊断准确性。影响诊断准确性。doi：http://dx.doi.org/10.12669/pjms.315.8411引用方式：Saba K，Niazi S，Bukhari MH，Imam SF。与番木瓜染色相比，使用上甲苯甲蓝染色可改善细针穿刺细胞学报告的效率。 Pak J Med Sci 2015; 31（5）：1146-1151。 doi：http：//dx.doi.org/10.12669/pjms.315.8411这是根据知识共享署名许可协议（http://creativecommons.org/licenses/by/3.0）的条款分发的开放获取文章，只要适当引用了原始作品，便可以在任何介质中不受限制地使用，分发和复制。

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