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首页> 外文期刊>Pathogens >The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays
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The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays

机译：超免疫鸡参照血清的使用不适用于流感假型中和试验的验证

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The pseudotype particle neutralization test (pp-NT) is a next-generation serological assay employed for the sensitive study of influenza antibody responses against hemagglutinin (HA), including stalk-directed antibodies. However, a validation of this assay has yet to be performed, and this limits its use to primarily research laboratories. To identify possible serological standards to be used in optimization and validation of the pp-NT, we have evaluated the cross-reactivity of hyperimmune chicken reference antisera in this assay. Our findings show that the cross-reactivity detected by the pp-NT is only partly explained by phylogenetic relationships and protein homology between the HA subtypes analysed; further studies are necessary to understand the origin of the cross-reactivity detected, and reference standards with higher specificity should be evaluated or generated de novo for future use in pp-NT.
机译：假型颗粒中和试验（pp-NT）是用于敏感研究流感病毒对血凝素（HA）包括茎定向抗体的抗体反应的下一代血清学检测方法。但是，该测定方法尚待验证，这将其用途限制在主要是研究实验室。为了确定可用于优化和验证pp-NT的可能的血清学标准，我们在此测定法中评估了超免疫鸡参考抗血清的交叉反应性。我们的发现表明，由pp-NT检测到的交叉反应性仅部分由分析的HA亚型之间的系统发育关系和蛋白质同源性解释;为了了解所检测到的交叉反应的起源，有必要进行进一步的研究，并且应评估或特异性更高的参考标准品，以备将来在pp-NT中使用。

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