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首页> 外文期刊>Pathogens >Pyrosequencing Reveals the Predominance of Pseudomonadaceae in Gut Microbiome of a Gall Midge
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Pyrosequencing Reveals the Predominance of Pseudomonadaceae in Gut Microbiome of a Gall Midge

机译：焦磷酸测序揭示了mon蚊肠道微生物组中假单胞菌科的优势。

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Gut microbes are known to play various roles in insects such as digestion of inaccessible nutrients, synthesis of deficient amino acids, and interaction with ecological environments, including host plants. Here, we analyzed the gut microbiome in Hessian fly, a serious pest of wheat. A total of 3,654 high quality sequences of the V3 hypervariable region of the 16S rRNA gene were obtained through 454-pyrosequencing. From these sequences, 311 operational taxonomic units (OTUs) were obtained at the ≥97% similarity cutoff. In the gut of 1st instar, otu01, a member of Pseudomonas, was predominant, representing 90.2% of total sequences. otu13, an unidentified genus in the Pseudomonadaceae family, represented 1.9% of total sequences. The remaining OTUs were each less than 1%. In the gut of the 2nd instar, otu01 and otu13 decreased to 85.5% and 1.5%, respectively. otu04, a member of Buttiauxella, represented 9.7% of total sequences. The remaining OTUs were each less than 1%. In the gut of the 3rd instar, otu01 and otu13 further decreased to 29.0% and 0%, respectively. otu06, otu08, and otu16, also three members of the Pseudomonadaceae family were 13.2%, 8.6%, and 2.3%, respectively. In addition, otu04 and otu14, two members of the Enterobacteriaceae family, were 4.7% and 2.5%; otu18 and otu20, two members of the Xanthomonadaceae family, were 1.3% and 1.2%, respectively; otu12, a member of Achromobacter, was 4.2%; otu19, a member of Undibacterium, was 1.4%; and otu9, otu10, and otu15, members of various families, were 6.1%, 6.3%, and 1.9%, respectively. The investigation into dynamics of Pseudomonas, the most abundant genera, revealed that its population level was at peak in freshly hatched or 1 day larvae as well as in later developmental stages, thus suggesting a prominent role for this bacterium in Hessian fly development and in its interaction with host plants. This study is the first comprehensive survey on bacteria associated with the gut of a gall midge, and provides a foundation for future studies to elucidate the roles of gut microbes in Hessian fly virulence and biology.
机译：肠道细菌在昆虫中扮演着各种角色，例如消化无法获取的养分，合成氨基酸不足以及与包括寄主植物在内的生态环境相互作用。在这里，我们分析了黑森州苍蝇（一种严重的小麦害虫）中的肠道微生物组。通过454-焦磷酸测序获得了总共3,654个高质量的16S rRNA基因的V3高变区序列。从这些序列中，获得了≥97％相似性截止值的311个操作分类单位（OTU）。在第一龄幼虫的肠道中，假单胞菌的成员otu01占主导地位，占总序列的90.2％。 otu13是假单胞菌科的一个未鉴定属，占总序列的1.9％。其余OTU分别小于1％。在第二龄幼虫的肠中，otu01和otu13分别降至85.5％和1.5％。 Buttiauxella的成员otu04占总序列的9.7％。其余OTU分别小于1％。在三龄肠中，otu01和otu13分别下降至29.0％和0％。 otu06，otu08和otu16也是假单胞菌科的三个成员，分别为13.2％，8.6％和2.3％。此外，肠杆菌科的两个成员otu04和otu14分别为4.7％和2.5％; Xanthomonadaceae家族的两个成员otu18和otu20分别为1.3％和1.2％;无色杆菌成员otu12为4.2％; Undibacterium的成员otu19为1.4％;各个家庭的成员otu9，otu10和otu15分别为6.1％，6.3％和1.9％。对最丰富的属假单胞菌的动力学研究表明，其种群水平在刚孵出的或1天的幼体以及后期的发育阶段处于高峰，因此表明该细菌在黑森州果蝇的发育及其繁殖中起着重要作用。与寄主植物的相互作用。这项研究是对与mid蚊肠道相关细菌的首次全面调查，并为今后研究阐明肠道微生物在黑森州蝇毒力和生物学中的作用提供了基础。

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