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Guidelines for collection and processing of lungs from aged mice for histological studies

机译：老年小鼠肺组织收集和处理指南

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Pulmonary inflammation and the development of spontaneous lung tumors are important age-related lesions in mice. Therefore, gross and histological examination of the respiratory system is a critical component of geropathology research studies for translating surrogate endpoints to clinical aging studies. Collection, trimming, and processing of lung tissue from aged mice require a high-quality sequential process since aged mice are irreplaceable resource-intensive animal models. This protocol provides a basic technique that provides excellent sections for histological evaluation of the respiratory system of old mice suitable for most research applications. The points of emphasis are infusing the lungs at necropsy with formalin through the airways to prevent atelectasis artifacts that can preclude accurate histological evaluation, and embedding of anatomically oriented in toto lung lobes to allow for complete and thorough evaluation of all lung regions.
机译：肺部炎症和自发性肺部肿瘤的发展是小鼠中与年龄相关的重要病变。因此，对呼吸系统的全面和组织学检查是将替代终点转换为临床衰老研究的病理学研究的关键组成部分。由于老年小鼠是不可替代的资源密集型动物模型，因此从老年小鼠收集，修剪和加工肺组织需要高质量的顺序过程。该协议提供了一种基础技术，可为适合大多数研究应用的老年小鼠呼吸系统的组织学评估提供出色的内容。重点是在尸检时向肺中注入福尔马林，以防止肺不张，从而无法进行准确的组织学评估，并在解剖学上将其嵌入肺叶内，以便对所有肺区域进行完整而彻底的评估。

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