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首页> 外文期刊>Parasites Vectors >Cold hardiness and biochemical response to low temperature of the unfed bush tick Haemaphysalis longicornis (Acari: Ixodidae)
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Cold hardiness and biochemical response to low temperature of the unfed bush tick Haemaphysalis longicornis (Acari: Ixodidae)

机译：未进食的布什tick虫的嗜血菌对低温的抗寒性和生化响应（Acari：Ixodidae）

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Background The survival of overwintering ticks, is critical for their subsequent population dynamics in the spring, and consequent transmission of tick-borne diseases. Survival is largely influenced by the severity of the winter temperatures and their degree of cold hardiness at the overwintering stage. The bush tick Haemaphysalis longicornis, is widely distributed in China, and can transmit various pathogens that pose serious medical/veterinary problems. In the present study we investigated the effect of low temperature stress to tick survival, super-cooling point and body content of water, glycerol and total protein. Methods After various temperature acclimations, the super-cooling point was measured by Ni/CrNi-thermocouples with a precision temperature recorder. Water content was determined from weight loss of the sample exposed to 60°C for 48?h. Glycerol content was determined using Free Glycerol Reagent as directed by the manufacturer, and total protein was determined using the Bradford assay. Results The 50% mortality temperatures for the adults and nymphs were -13.7°C and -15.2°C, respectively; and the discriminating temperatures for the adults and nymphs were -16.0°C and -17.0°C, respectively. The super-cooling points of the adults and nymphs were -19.0°C and -22.7°C, respectively. The water content of adult H. longicornis decreased substantially after acclimation at 0°C for 10 d, whereas the nymphs decreased after acclimation at 0°C for 20 d, and the glycerol and proteins of both nymphs and adults were significantly increased (p?
机译：背景越冬survival的生存对春季随后的种群动态以及of传播疾病的传播至关重要。生存很大程度上受冬季温度的严重性及其在越冬阶段的耐寒性影响。布什tick虫在中国广泛分布，可以传播各种严重的医学/兽医问题的病原体。在本研究中，我们研究了低温胁迫对tick生存，过冷点和体内水，甘油和总蛋白含量的影响。方法经过各种温度适应后，用精密温度记录仪通过Ni / CrNi热电偶测量过冷点。由暴露于60°C 48？h的样品的失重确定水分含量。使用游离甘油试剂按照制造商的指示确定甘油含量，并使用Bradford分析确定总蛋白。结果成人和若虫的50％死亡率分别为-13.7°C和-15.2°C。成虫和若虫的鉴别温度分别为-16.0°C和-17.0°C。成虫和若虫的过冷点分别为-19.0°C和-22.7°C。成虫H. longicornis的水分在0°C适应10 d后显着降低，而若虫在0°C适应20 d后降低，并且若虫和成虫的甘油和蛋白质均显着增加（p？ <？0.01）在0°C应力10 d时。结论在H. longicornis中，低温胁迫可以增强其抗寒性并引发适当的响应，包括降低水分含量，增加甘油和总蛋白含量。

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