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首页> 外文期刊>Sensing and Bio-Sensing Research >Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region {CA1} of hippocampal slices with a glass capillary-based l-glutamate sensor
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Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region {CA1} of hippocampal slices with a glass capillary-based l-glutamate sensor

机译：基于玻璃毛细管的l-谷氨酸传感器实时监测海马切片{CA1}区域高频刺激释放的细胞外l-谷氨酸水平

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Abstract Real-time monitoring of l-glutamate released by high-frequency stimulation in region {CA1} of mouse hippocampal slices was performed with a glass capillary-based sensor, in combination with the recoding of excitatory postsynaptic potentials (fEPSPs). A method for extracting l-glutamate currents from the recorded ones was described and applied for determining the level of extracellular l-glutamate released by 100 Hz stimulation. Recording of an l-glutamate current with a current sampling interval of 1 Hz was found to be useful for acquiring a Faradaic current that reflects l-glutamate level released by the high-frequency stimulation of 7 trains, each 20 stimuli at 100 Hz and inter-train interval of 3 s. The l-glutamate level was obtained as 15 ± 6 μM (n = 8) for the persistent enhancement of fEPSPs, i.e., the induction of long-term potentiation (LTP), and 3 ± 1 μM (n = 5) for the case of no {LTP} induction. Based on these observations, the level of the extracellular l-glutamate was shown to play a crucial role in the induction of LTP.
机译：摘要利用玻璃毛细管为基础的传感器，结合对兴奋性突触后电位（fEPSPs）的记录，对小鼠海马切片{CA1}区域中高频刺激释放的l-谷氨酸进行了实时监测。描述了一种从记录的电流中提取l-谷氨酸电流的方法，并将其应用于确定由100Hz刺激释放的细胞外1-谷氨酸的水平。发现以1 Hz的电流采样间隔记录l-谷氨酸电流可用于获取法拉第电流，该电流反映法拉第电流，该电流反映了7列高频刺激释放的l-谷氨酸水平，每列20刺激分别在100 Hz和200 Hz -火车间隔为3 s。对于fEPSP的持续增强，即诱导长期增强（LTP），获得的l-谷氨酸水平为15±6μM（n = 8），在这种情况下，为3±1μM（n = 5）没有{LTP}归纳法。基于这些观察结果，表明细胞外L-谷氨酸的水平在LTP的诱导中起关键作用。

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