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首页> 外文期刊>Pharmacognosy Research >Modulation of genotoxicity of oxidative mutagens by glycyrrhizic acid from Glycyrrhiza glabra L.
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Modulation of genotoxicity of oxidative mutagens by glycyrrhizic acid from Glycyrrhiza glabra L.

机译：甘草中的甘草酸调节氧化性诱变剂的遗传毒性。

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Background:The chemopreventive effects of certain phytoconstituents can be exploited for their use as functional foods, dietary supplements and even as drugs. The natural compounds, acting as anti-genotoxic and free radical scavenging compounds, may serve as potent chemopreventive agents. These can inhibit DNA modulatory activities of mutagens and help preventing pathological processes.Objectives:Present study on Glycyrrhiza glabra L., a promising medicinal plant, widely used in traditional medicine, focused on the bioassay-guided fractionation of its extracts for the isolation of certain phytochemicals with anti-genotoxic potential against oxidative mutagens.Materials and Methods:The methanol extract of Glycyrrhiza glabra rhizomes was subjected to column chromatography, and isolated fraction was evaluated for its anti-genotoxic and antioxidant potential using SOS chromotest, Comet assay, and DPPH radical scavenging assay.Results:GLG fraction, which was characterized as Glycyrrhizic acid, inhibited the genotoxicity of oxidative mutagens viz., H2O2 and 4NQOquite efficiently. In SOS chromotest, using E.coli PQ37 tester strain, it inhibited induction factor induced by H2O2 and 4NQO by 75.54% and 71.69% at the concentration of 121.46 μM,respectively. In Comet assay, it reduced the tail moment induced by H2O2 and 4NQO by 70.21% and 69.04%, respectively, at the same concentration in human blood lymphocytes. The isolated fraction also exhibited DPPH free radical scavenging activity and was able to scavenge 85.95% radicals at a concentration of 120 μM.Conclusion:Glycyrrhizic acid is a potential modulator of genotoxins as well as efficient scavenger of free radicals.
机译：背景：某些植物成分的化学预防作用可被用作功能性食品，膳食补充剂甚至药物。天然化合物可作为抗基因毒性和清除自由基的化合物，可以用作有效的化学预防剂。这些可以抑制诱变剂的DNA调节活性，并有助于防止病理过程。目的：目前对甘草（Glycyrrhiza glabra L.）的研究是一种很有前景的药用植物，广泛用于传统医学中，致力于将其提取物进行生物测定指导分离，以分离某些对氧化诱变剂具有抗遗传毒性的植物化学物质。方法：对甘草根茎的甲醇提取物进行柱色谱分析，并使用SOS色度测试，彗星分析和DPPH自由基评估分离出的部分的抗遗传毒性和抗氧化能力。结果：以甘草酸为特征的GLG馏分有效抑制了氧化诱变剂，H2O2和4NQOquite的遗传毒性。在SOS色谱测试中，使用大肠杆菌PQ37测试菌株，在121.46μM的浓度下，它分别抑制了H2O2和4NQO诱导的诱导因子75.54％和71.69％。在彗星试验中，在相同浓度的人血淋巴细胞中，H2O2和4NQO诱导的尾部矩分别降低了70.21％和69.04％。分离出的级分还具有清除DPPH自由基的活性，并能清除浓度为120μM的85.95％的自由基。结论：甘草酸是一种潜在的基因毒素调节剂，同时也是清除自由基的有效方法。

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