• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pharmacognosy Research >Anti-Advanced Glycation End-product and Free Radical Scavenging Activity of Plants from the Yucatecan Flora
【24h】

Anti-Advanced Glycation End-product and Free Radical Scavenging Activity of Plants from the Yucatecan Flora

机译：Yucatecan植物区系的植物抗高级糖基化终产物和自由基清除活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Formation and accumulation of advanced glycation end-products (AGE) is recognized as a major pathogenic process in diabetic complications, atherosclerosis and cardiovascular diseases. In addition, reactive oxygen species and free radicals have also been reported to participate in AGE formation and in cell damage. Natural products with antioxidant and antiAGE activity have great therapeutic potential in the treatment of diabetes, hypertension and related complications. Objective: to test ethanolic extracts and aqueous-traditional preparations of plants used to treat diabetes, hypertension and obesity in Yucatecan traditional medicine for their anti-AGE and free radical scavenging activities. Materials and Methods: ethanolic extracts of leaves, stems and roots of nine medicinal plants, together with their traditional preparations, were prepared and tested for their anti-AGE and antioxidant activities using the inhibition of advanced glycation end products and DPPH radical scavenging assays, respectively. Results: the root extract of C. fistula (IC50= 0.1 mg/mL) and the leaf extract of P. auritum (IC50= 0.35 mg/mL) presented significant activity against vesperlysine and pentosidine-like AGE. Although none of the aqueous traditional preparations showed significant activity in the anti-AGE assay, both the traditional preparations and the ethanolic extracts of E. tinifolia, M. zapota, O. campechianum and P. auritum showed significant activity in the DPPH reduction assay. Conclusions: the results suggest that the metabolites responsible for the detected radical-scavenging activity are different to those involved in inhibiting AGE formation; however, the extracts with antioxidant activity may contain other metabolites which are able to prevent AGE formation through a different mechanism. SUMMARY Ethanolic extracts from nine plants used to treat diabetes, hypertension and obesity in Yucatecan traditional medicine were tested for their anti-AGE and free radical scavenging activities. Significant activity against vesperlysine and pentosidine-like AGE was detected in the root extract of Cassia fistula and the leaf extract of Piper auritum . Traditional preparations and the ethanolic extracts of Ehretia tinifolia, Manilkara zapota, Ocimum campechianum and Piper auritum showed significant activity in the DPPH reduction assay. Results suggest that the metabolites responsible for the detected radical-scavenging activity are different to those involved in inhibiting AGE formation. Abbreviations Used : AGE: Advanced glycation end-product; DPPH: 2,2-Diphenyl-1-picrylhydrazyl; DM: Diabetes mellitus; ROS: Reactive oxygen species; BSA: Bovine serum albumin; EtOH: Ethanol; EtOAc: Ethyl acetate; ANOVA: Analysis of variance; BA: Brosimum alicastrum ; BS: Bunchosia swartziana ; CF: Cassia fistula ; CN: Cocos nucifera ; ET: Ehretia tinifolia ; MZ: Manilkara zapota ; OC: Ocimum campechianum ; PA: Piper auritum ; RM: Rhizophora mangle ; L: Leaves; S: Stems; R: Roots; T: traditional preparation; I: Inflorescences; W: Water Key words: Antioxidant activity, diabetes, glycation-end products, traditional medicine.
机译：背景：晚期糖基化终产物（AGE）的形成和积累被认为是糖尿病并发症，动脉粥样硬化和心血管疾病的主要致病过程。此外，据报道活性氧和自由基也参与了AGE的形成和细胞损伤。具有抗氧化和抗AGE活性的天然产物在糖尿病，高血压和相关并发症的治疗中具有巨大的治疗潜力。目的：检测尤卡坦传统药物中用于治疗糖尿病，高血压和肥胖症的乙醇提取物和水传统制剂的抗衰老和清除自由基的活性。材料和方法：制备了九种药用植物的叶，茎和根的乙醇提取物，以及它们的传统制剂，并分别使用高级糖化终产物的抑制作用和DPPH自由基清除试验测试了它们的抗衰老和抗氧化活性。 。结果：呈现了瘘管的根提取物（IC 50 = 0.1 mg / mL）和金缕梅的叶提取物（IC 50 = 0.35 mg / mL）。具有明显的抗vesperlysine和戊糖苷样AGE的活性。尽管没有一种含水的传统制剂在抗AGE试验中显示出显着的活性，但是传统的制剂和E. tinifolia，M。zapota，O。campechianum和P. auritum的乙醇提取物在DPPH还原试验中都显示出了显着的活性。结论：结果表明，负责检测自由基清除活性的代谢产物与抑制AGE形成的代谢产物不同。但是，具有抗氧化活性的提取物可能含有其他代谢物，这些代谢物能够通过不同的机制阻止AGE的形成。总结测试了来自Yucatecan传统药物中用于治疗糖尿病，高血压和肥胖症的9种植物的乙醇提取物的抗衰老和清除自由基的活性。在决明子的根部提取物和金合欢的叶提取物中检测到了显着的抗vesperlysine和戊糖苷样AGE活性。传统的制剂和薄荷叶大黄菊，马尼卡拉杂种，樟子松和Pipe木的乙醇提取物在DPPH还原试验中显示出显着的活性。结果表明，负责检测自由基清除活性的代谢产物与抑制AGE形成的代谢产物不同。使用的缩写：年龄：高级糖化终产物; DPPH：2，2-二苯基-1-吡啶并肼基; DM：糖尿病; ROS：活性氧; BSA：牛血清白蛋白;乙醇：乙醇;乙醇。 EtOAc：乙酸乙酯;乙酸乙酯。方差分析：方差分析; BA：拟南芥; BS：凤尾草; CF：决明子瘘; CN：椰子（Cocos nucifera）; ET：Ehretia tinifolia; MZ：Manilkara zapota; OC：Ocimum campechianum; PA：风笛耳; RM：根瘤菌; L：叶子; S：茎; R：根源; T：传统的准备; I：花序; W：水关键词：抗氧化活性，糖尿病，糖基化终产物，传统药物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号