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首页> 外文期刊>Pharmacognosy Research >Anti-inflammatory Activity of Berry Fruits in Mice Model of Inflammation is Based on Oxidative Stress Modulation
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Anti-inflammatory Activity of Berry Fruits in Mice Model of Inflammation is Based on Oxidative Stress Modulation

机译：浆果在小鼠炎症模型中的抗炎活性基于氧化应激调节

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Background: Many fruits have been used as nutraceuticals because the presence of bioactive molecules that play biological activities. Objective: The present study was designed to compare the anti-inflammatory and antioxidant effects of methanolic extracts of Lycium barbarum (GOJI), Vaccinium macrocarpon (CRAN) and Vaccinium myrtillus (BLUE). Materials and Methods: Mices were treated with extracts (50 and 200 mg/kg, p.o.), twice a day through 10 days. Phytochemical analysis was performed by high-performance liquid chromatography. Antioxidant activity was determine by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, reducing power, lipid peroxidation thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and catalase (CAT) activity. Anti-inflammatory activity was evaluated by paw edema followed by determination of myeloperoxidase (MPO) and TBARS. Results: High amount of phenolic compounds, including rutin, were identified in all berries extracts. However, quercetin was observed only in BLUE and CRAN. GOJI presents higher scavenging activity of DPPH radical and reducing power than BLUE and CRAN. The extracts improved antioxidant status in liver; BLUE showed the largest reduction (75.3%) in TBARS when compared to CRAN (70.7%) and GOJI (65.3%). Nonetheless, CAT activity was lower in BLUE group. However, hepatic concentrations of GSH were higher in animals treated with GOJI rather than CRAN and BLUE. Despite all fruits caused a remarkable reduction in paw edema and TBARS, only BLUE and CRAN were able to reduce MPO. Conclusion: These results suggest that quercetin, rutin, or other phenolic compound found in these berry fruits extracts could produce an anti-inflammatory response based on modulation of oxidative stress in paw edema model. SUMMARY Within fruits broadly consumed because of its nutraceuticals properties include, Lycium barbarum (Goji berry), Vaccinium myrtillus (Blueberry or Bilberry) and Vaccinium macrocarpon (Cranberry) The objectives of this study were the investigation and comparison of chemical composition, antioxidant activity “ in vitro ” and “ in vivo ” and anti inflammatory property of berry fruits bought dry form. In summary, two main findings can be addressed with this study: (1) Berry fruits presented antioxidant and anti inflammatory activities “ in vitro ” and “ in vivo ”; (2) the extracts of GOJI, CRAN, and BLUE modulate the inflammatory process by different mechanisms.
机译：背景：许多水果已被用作营养保健品，因为存在着具有生物活性的生物活性分子。目的：本研究旨在比较枸杞（GOJI），大果越橘（CRAN）和黑豆越橘（BLUE）的甲醇提取物的抗炎和抗氧化作用。材料和方法：Mi两次用提取物（50和200 mg / kg，口服）治疗，每天两次，持续10天。通过高效液相色谱法进行植物化学分析。抗氧化剂活性通过2,2-二苯基-1-吡啶并肼（DPPH）测定，还原力，脂质过氧化硫代巴比妥酸反应性物质（TBARS），谷胱甘肽（GSH）和过氧化氢酶（CAT）活性来确定。通过爪水肿，然后测定髓过氧化物酶（MPO）和TBARS来评估抗炎活性。结果：在所有浆果提取物中均鉴定出大量酚类化合物，包括芦丁。但是，槲皮素仅在BLUE和CRAN中观察到。与蓝色和CRAN相比，GOJI具有更高的DPPH自由基清除活性和降低的功率。提取物改善了肝脏的抗氧化状态;与CRAN（70.7％）和GOJI（65.3％）相比，BLUE的TBARS减少幅度最大（75.3％）。但是，BLUE组的CAT活性较低。但是，用GOJI而非CRAN和BLUE治疗的动物肝脏中GSH的浓度较高。尽管所有水果均导致爪水肿和TBARS显着降低，但只有BLUE和CRAN能够降低MPO。结论：这些结果表明，在这些浆果提取物中发现的槲皮素，芦丁或其他酚类化合物可通过调节爪水肿模型中的氧化应激产生抗炎反应。小结在由于其营养保健特性而广泛食用的水果中，包括枸杞（枸杞），牛痘越桔（蓝莓或越桔）和大果越橘（蔓越莓）。本研究的目的是调查和比较其中的化学成分，抗氧化活性浆果的“体外”和“体内”及抗炎特性以干燥形式购得。总之，这项研究可以解决两个主要发现：（1）浆果在“体外”和“体内”具有抗氧化和抗炎活性; （2）GOJI，CRAN和BLUE的提取物通过不同的机制调节炎症过程。

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