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首页> 外文期刊>Pharmacognosy Research >Extracts from Flammulina velutipes Inhibit the Adhesion of Pathogenic Fungi to Epithelial Cells
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Extracts from Flammulina velutipes Inhibit the Adhesion of Pathogenic Fungi to Epithelial Cells

机译：金针菇提取物抑制病原真菌对上皮细胞的粘附

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Background: Recently, extracts from natural sources have been tested for their antifungal properties. In this aspect, Flammulina velutipes extracts possess a significant amount of branch-chained carbohydrates with mannose moieties that, hypothetically, can reduce the adhesion. Objective: In this study, we assessed the capacity of extracts from F. velutipes (wild-type AQF-1 and ATCC 34574 as the reference strain) to inhibit the adhesion of S. schenkii and C. albicans to epithelial cells. Materials and Methods: The aqueous extracts from F. velutipes strains were obtained by sonication, total carbohydrate and protein was analyzed by Dubois and Lowry methods respectively. Effect of the extracts (50, 100 and 150 μg/mL) on the fungi adhesion to host cells was evaluated after 1 h interaction, and the percentage of inhibition of adhesion was measured. After of interaction the cytoskeleton from cell was analyzed with phalloidin-FITC. Results: The extract from strain AQF-1 (50, 100 and 150 μg/mL) inhibited the adhesion of: S. schenkii in a dose-dependent manner (4.9, 7.5 and 12.7%, respectively) and C. albicans in a dose-independent manner (5.2%). The percentage of inhibition by extracts from the strain ATCC34574 at the same concentrations, shown that are dose independent for both fungi: 3.9% for S. schenkii and 2.6% for C. albicans . Conclusion: The extracts from F. velutipes inhibit the adhesion of pathogenic fungi to host cells. The mechanism molecular is unknown; however, is probably an interaction between the polysaccharides from extracts with the fungi receptors. This aspect is currently analyzed. SUMMARY The yields of mycelium from two strains of F. velutipes and the extract from it were similar. Extracts from both strains have inhibited adhesion of S. schenkii and C. albicans to epithelial cells in vitro , but the extract from strain AQF-1 was more effective. The extracts have not prevented damage to epithelial cells caused by pathogenic fungi. Abbreviation Used: YPG: Yeast peptone glucose, DMEM: Dulbecco's Modified Eagle's medium, FITC: Fluorescein isothiocyanate.
机译：背景：最近，已经对来自天然来源的提取物的抗真菌特性进行了测试。在这方面，金针菇提取物具有大量的支链碳水化合物和甘露糖部分，假设可以减少粘附。目的：在这项研究中，我们评估了金针菇提取物（野生型AQF-1和ATCC 34574作为参考菌株）抑制S. schenkii和白色念珠菌粘附于上皮细胞的能力。材料与方法：超声处理获得金针菇菌株的水提物，分别用Dubois法和Lowry法分析总碳水化合物和蛋白质。相互作用1小时后评估提取物（50、100和150μg/ mL）对真菌对宿主细胞粘附的影响，并测量粘附抑制的百分比。相互作用后，用鬼笔环肽-FITC分析细胞的细胞骨架。结果：菌株AQF-1的提取物（50、100和150μg/ mL）以剂量依赖性方式（分别为4.9、7.5和12.7％）和白色念珠菌对剂量的S. schenkii和白色念珠菌具有抑制作用。 -独立方式（5.2％）。在相同浓度下，菌株ATCC34574的提取物的抑制百分比显示出对两种真​​菌均不受剂量影响：S。schenkii为3.9％，白色念珠菌为2.6％。结论：金针菇提取物抑制病原真菌与宿主细胞的粘附。分子机制尚不清楚;然而，可能是提取物中的多糖与真菌受体之间的相互作用。当前对此方面进行了分析。发明内容来自两种天鹅绒镰刀菌的菌丝体的产量及其提取物是相似的。两种菌株的提取物均在体外抑制了S. schenkii和白色念珠菌对上皮细胞的粘附，但菌株AQF-1的提取物更有效。提取物尚未阻止病原性真菌对上皮细胞的损害。使用的缩写：YPG：酵母蛋白glucose葡萄糖，DMEM：Dulbecco改良的Eagle培养基，FITC：异硫氰酸荧光素。

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