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首页> 外文期刊>Pharmacognosy Research >Enhanced Synthesis of Curculigoside by Stress and Amino Acids in Static Culture of Curculigo orchioides Gaertn (Kali Musli)
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Enhanced Synthesis of Curculigoside by Stress and Amino Acids in Static Culture of Curculigo orchioides Gaertn (Kali Musli)

机译：在静态培养的兰茅（Caliculo orchioides Gaertn（Kali Musli））中，通过胁迫和氨基酸增强了兰茅苷的合成

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Background: Curculigo orchioides Gaertn (Kali musli; Family: Hypoxidaceae) is an endangered medicinal plant used for many medicinal purposes such as impotency, aphrodisiac, tonic, jaundice, and skin ailments. Its hepatoprotective, antioxidant, and anti-cancerous potential have also been evaluated by many scientists. Objective: The objective of this study is to enhance the curculigoside content in tissue culture of C. orchioides . Materials and Methods: The present study deals with the enhancement of an active compound of C. orchioides by incorporating various concentration of phenylalanine (Phe), tyrosine, (20, 40, 60, and 80 mg/100 ml), chromium (Cr) and nickel (Ni) (1, 2, 3, 4, and 5 ppm) into Zenk media in controlled and aseptic conditions. Results: Plant secondary metabolites are unique sources for pharmaceuticals, food additives, flavors, and industrially important biochemicals. Accumulation of such metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. A significantly remarkable enhancement in all induced samples was noted. Curculigoside content was maximum in the 6-week-old tissue induced with 3 ppm of Cr (7.63%) followed by 4 weeks tissue of tissue fed with 4 ppm of Ni (5.66%) and 4-week-old tissue fed with tyrosine 7.5 mg/100 ml (2.38%) among all samples used. These results suggest that tyrosine is better enhancer than Phe in the biosynthetic pathway of curculigoside. The presence of curculigoside in all extracts was confirmed by Fourier transform infrared spectroscopy, high-performance thin layer chromatography analysis with standard compound of curculigoside and histology of treated samples. Conclusion: This investigation was carried out for the 1st time, and it is a significant step in understanding the biochemistry of curculigoside. The developed protocol will be beneficial for marketing in pharmaceutical industries. SUMMARY Curculigo orchioides Gaertn (Kali musli; Family: Hypoxidaceae) is an endangered medicinal plant used for many medicinal purposes such as impotency, aphrodisiac, tonic, jaundice, and skin ailments. It was observed that dry matter % was maximum in 6-week-old tissue fed with 2.5 mg/100 ml of tyrosine and diminished beyond this concentration among all samples used The nickel (Ni) and chromium (Cr) stress has enhanced the curculigoside in considerable amount in nontoxic range, in tissue culture of C. orchioides . Curculigoside content was maximum in 6-week-old tissue induced with 3 ppm of Cr (7.63%; 11-fold enhancement) followed by 4 weeks tissue of tissue fed with 4 ppm of Ni (5.66%) and 4-week-old tissue fed with tyrosine 7.5 mg/100 ml (2.38%) among all samples used. Histological studies confirmed the enhanced production of curculigoside. Abbreviations Used: Phe: Phenylalanine, PAL: Phenylalanine ammonia-lyase, mM: mille Molar, Cr: Chromium, Ni: Nickel, HPTLC: High-performance thin layer chromatography.
机译：背景：Curculigo orchioides Gaertn（卡利musli;家族：Hypoxidaceae）是一种濒临灭绝的药用植物，可用于多种医学用途，例如壮阳，壮阳药，滋补品，黄疸病和皮肤病。许多科学家还评估了它的保肝，抗氧化和抗癌潜力。目的：本研究的目的是提高兰花C科植物组织培养物中仙茅苷的含量。材料和方法：本研究通过掺入各种浓度的苯丙氨酸（Phe），酪氨酸，（20、40、60和80 mg / 100 ml），铬（Cr）来增强兰花C虫的活性化合物。在受控和无菌条件下将镍（Ni）（1、2、3、4和5 ppm）注入Zenk介质中。结果：植物次生代谢产物是药品，食品添加剂，调味剂和重要的工业生化试剂的独特来源。此类代谢产物的积累经常发生在承受各种胁迫（包括各种激发子或信号分子）的植物中。注意到所有诱导样品中的显着显着增强。在3周龄的Cr（7.63％）诱导的6周龄组织中，姜黄素含量最高，其次是4周龄的组织中饲喂4 ppm的镍（5.66％）和4周龄的组织中酪氨酸7.5在所有使用的样品中，mg / 100 ml（2.38％）。这些结果表明，在仙茅苷的生物合成途径中，酪氨酸比苯丙氨酸更好。通过傅里叶变换红外光谱法，使用标准药草苷的化合物进行的高效薄层色谱分析和处理样品的组织学证实了所有提取物中均存在药草苷的存在。结论：这项研究是第一次进行的，这是理解仙茅苷的生物化学的重要一步。开发的协议将有利于制药行业的营销。发明内容兰茅草（Kalculigo orchioides Gaertn）（卡利（Kali musli）;家族：Hypoxidaceae）是一种濒临灭绝的药用植物，可用于许多医学用途，例如壮阳，壮阳药，补品，黄疸病和皮肤病。观察到，在使用2.5 mg / 100 ml酪氨酸的6周龄组织中，干物质％最高，并且在所有使用的样品中均降低到该浓度以下。镍（Ni）和铬（Cr）胁迫增强了仙茅中的仙茅苷。在无毒范围内，对拟南芥的组织培养相当可观。在3 ppm的Cr（7.63％; 11倍增强）诱导的6周龄组织中，仙茅苷的含量最高，随后是4周组织的4 ppm Ni（5.66％）和4周龄的组织在所有使用的样品中均添加了7.5 mg / 100 ml（2.38％）的酪氨酸。组织学研究证实了仙茅苷的产量增加。使用的缩写：Phe：苯丙氨酸，PAL：苯丙氨酸氨解酶，mM：Mille Molar，Cr：铬，Ni：镍，HPTLC：高效薄层色谱。

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