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首页> 外文期刊>Physiological Reports >Effects of maternal western‐style diet on amniotic fluid volume and amnion VEGF profiles in a nonhuman primate model
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Effects of maternal western‐style diet on amniotic fluid volume and amnion VEGF profiles in a nonhuman primate model

机译：母体西式饮食对非人类灵长类动物模型中羊水量和羊膜VEGF谱的影响

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During pregnancy, high fat diet (HFD) induces maternal obesity, insulin resistance, and placental inflammatory responses that compromise placental and fetal development. Whether maternal HFD would adversely affect amniotic fluid volume (AFV) has not been explored. Vascular endothelial growth factor (VEGF) is expressed in the amnion and has been proposed as a regulator of AFV. Our aim was to investigate the effects of HFD on AFV and the associated changes in VEGF and soluble VEGF receptor 1 (sFlt‐1) expression profiles in three amnion regions of a nonhuman primate model. Further, we examined the relationships between VEGF expression and HFD‐induced changes in maternal metabolic status. Japanese macaques were maintained on control or HFD and amniotic fluid index (AFI) was measured as an ultrasonic estimate of AFV. Amniotic fluid VEGF concentrations were determined by ELISA and amnion VEGF and sFlt‐1 mRNA levels by real‐time RT‐qPCR. HFD increased maternal plasma triglyceride while glucose levels were unchanged. Maternal weight gain was found in diet‐sensitive animals whereas amniotic fluid VEGF concentration was reduced in diet‐resistant animals. HFD did not alter AFI and there was no correlation between AFI and maternal weight or amniotic fluid VEGF concentrations. VEGF mRNA levels were lowest in secondary placental amnion while sFlt‐1 mRNA were lowest in the primary placental amnion. HFD did not affect amnion VEGF or sFlt‐1 mRNA expression. These findings suggest that although maternal HFD increased maternal weight in diet‐sensitive and reduced amniotic fluid VEGF concentrations in diet‐resistant phenotype, AFV as indicated by the AFI, was not significantly affected.
机译：在怀孕期间，高脂饮食（HFD）会引起孕妇肥胖，胰岛素抵抗和胎盘炎症反应，从而损害胎盘和胎儿的发育。尚未探讨母体HFD是否会对羊水量（AFV）产生不利影响。血管内皮生长因子（VEGF）在羊膜中表达，并被提议作为AFV的调节剂。我们的目的是研究HFD对AFV的影响以及非人类灵长类动物模型三个羊膜区域中VEGF和可溶性VEGF受体1（sFlt-1）表达谱的相关变化。此外，我们检查了VEGF表达与HFD诱导的孕产妇代谢状态变化之间的关系。将日本猕猴维持在对照或HFD上，并测量羊水指数（AFI）作为AFV的超声估计。 ELISA测定羊水VEGF浓度，实时RT-qPCR测定羊膜VEGF和sFlt-1 mRNA水平。 HFD增加孕妇血浆甘油三酸酯，而血糖水平未改变。在饮食敏感的动物中发现产妇体重增加，而在饮食抗性动物中羊水VEGF浓度降低。 HFD不会改变AFI，AFI与孕妇体重或羊水VEGF浓度之间没有相关性。 VEGF在第二胎盘羊膜中的水平最低，而sFlt-1 mRNA在第一胎盘羊膜中的最低水平。 HFD不会影响羊膜VEGF或sFlt-1 mRNA的表达。这些发现表明，尽管母体HFD在饮食敏感性表型中增加了对饮食敏感的母亲体重，并降低了羊水中的VEGF浓度，但AFI指示的AFV并未受到明显影响。

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