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首页> 外文期刊>Physiological Reports >Epinephrine augments posttetanic potentiation in mouse skeletal muscle with and without myosin phosphorylation
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Epinephrine augments posttetanic potentiation in mouse skeletal muscle with and without myosin phosphorylation

机译：肾上腺素增强和不增强肌球蛋白磷酸化在小鼠骨骼肌中的强直性增强。

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Sympathetic tone may influence force potentiation, that is, the stimulation‐induced increase in skeletal muscle mechanical function associated with myosin phosphorylation, although the mechanism for this effect remains unknown. The purpose of this study was to examine the influence of epinephrine on concentric twitch force potentiation of wild‐type and skeletal myosin light‐chain kinase devoid mouse muscle (skMLCK ?/? ). To this end, concentric twitch force was assessed before and after a potentiating stimulus (PS) to determine the peak and the duration of potentiation in the absence (?EPI) and presence (+EPI) of 1? μ mol/L epinephrine in both genotypes. Twitch force of wild‐type and skMLCK ?/? muscles was increased by up to 31 and 35% and 18 and 23% in the ?EPI and EPI conditions, respectively (all data n ?=?8, P ??0.05). In wild‐type muscles, the PS increased RLC phosphorylation from 0.14?±?0.05 (rest) to 0.66?±?0.08?mol phos mol RLC; by 480?sec RLC phosphorylation had returned to baseline (all data n ?=?4 each time point, P ??0.05). Neither resting nor peak levels of RLC phosphorylation were altered by +EPI, although the duration of RLC phosphorylation was prolonged. In skMLCK ?/? muscles, RLC phosphorylation was not elevated above constituent levels by stimulation in either the ?EPI or +EPI condition. Thus, given the similarity in potentiation responses between genotypes our data suggest that the influence of epinephrine on potentiation was independent of skMLCK catalyzed phosphorylation of the RLC, although the clinical significance of this pathway for skeletal muscle function remains to be identified.
机译：交感神经张力可能影响力量增强，即与肌球蛋白磷酸化有关的骨骼肌机械功能受刺激诱导的增加，尽管这种作用的机制尚不清楚。这项研究的目的是研究肾上腺素对缺乏小鼠肌肉的野生型和骨骼肌肌球蛋白轻链激酶同心抽搐增强的影响。为此，在加强刺激（PS）之前和之后评估同心抽力，以确定在不存在（ΔEPI）和存在（+ EPI）为1π时加强的峰值和持续时间。两种基因型的μmol / L肾上腺素。野生型和skMLCK？/？的拉力在？EPI和EPI条件下，肌肉分别增加了31％和35％，18和23％（所有数据n = 8，P <0.05）。在野生型肌肉中，PS将RLC磷酸化从0.14？±？0.05（静止）增加到0.66？±？0.08？mol phos mol RLC;到480秒时，RLC的磷酸化已恢复到基线（每个时间点的所有数据n≤= 4，P≤<0.05）。 + EPI不会改变RLC磷酸化的静止水平或峰值水平，尽管RLC磷酸化的持续时间延长了。在skMLCK中？在肌肉中，在？EPI或+ EPI条件下，通过刺激，RLC磷酸化均未升高至高于组成水平。因此，鉴于基因型之间增强反应的相似性，我们的数据表明肾上腺素对增强的影响独立于skMLCK催化的RLC磷酸化，尽管该途径对骨骼肌功能的临床意义尚待确定。

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