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Responses to flooding stress in soybean seedlings with the alcohol dehydrogenase transgene

机译：乙醇脱氢酶转基因对大豆幼苗水淹胁迫的响应

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The soybean is relatively sensitive to disturbances arising from flooding before its emergence from the soil. When young soybean seedlings at an early stage are transferred to flooding anaerobic conditions, alcohol dehydrogenase ( Adh ) mRNA and Adh protein increase temporarily in the root tips, where active cell division demands high energy production. Since there is little information on the significance of the up-regulation of Adh for the tolerance of soybeans to flooding stress, we examined the response to flooding in transgenic soybean lines in which the soybean Adh ( GmAdh2 ) gene was introduced under the control of a constitutive promoter. Acquired transgenic soybean seeds from one out of 14 transgenic lines were subjected to flooding stress. Growth inhibition of soybean seedlings caused by flooding stress was reduced in soybeans with the GmAdh2 transgene. Protein analysis and enzyme assay at the early stage of growth of the soybean seedlings confirmed that Adh expressions and activities in transgenic soybeans were increased compared to control soybeans. These results indicated that the introduced GmAdh2 gene might have induced some change in glycolysis and alcohol fermentation, and improved the germination of transgenic soybeans under flooding stress.
机译：大豆对从土壤中涌出之前的洪水所引起的干扰相对敏感。当早期的年轻大豆幼苗转移到淹水的厌氧条件下时，乙醇脱氢酶（Adh）mRNA和Adh蛋白在根尖中会暂时增加，而活跃的细胞分裂需要高能量产生。由于关于Adh上调对大豆对水淹胁迫的耐受性的重要性的信息很少，因此我们研究了转基因大豆品系中对水淹的响应，在转基因大豆品系中，大豆Adh（GmAdh2）基因是在a调控下引入的。组成型启动子。从14个转基因品系中的1个获得的转基因大豆种子遭受水淹胁迫。带有GmAdh2转基因的大豆降低了淹水胁迫对大豆幼苗的生长抑制作用。大豆幼苗生长早期的蛋白质分析和酶分析证实，与对照大豆相比，转基因大豆中Adh的表达和活性增加。这些结果表明，导入的GmAdh2基因可能引起了糖酵解和酒精发酵的某些变化，并改善了水淹胁迫下转基因大豆的发芽率。

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