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首页> 外文期刊>Plant Protection Science >Distribution and molecular identification of Meloidogyne spp. parasitising flue-cured tobacco in Yunnan, China
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Distribution and molecular identification of Meloidogyne spp. parasitising flue-cured tobacco in Yunnan, China

机译：根结线虫属的分布和分子鉴定。寄生在中国云南的烤烟

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摘要

Twenty-one populations of root-knot nematodes (RKNs) were recovered from diseased roots collected from flue-cured tobacco in 21 locations in Yunnan (China) during 2014–2015. Molecular diagnosis on species was performed based on characteristics of sequences of D2-D3 expansion domains of the 28S rDNA and sequence characterised amplified regions (SCAR). SCAR results identified 13 populations as Meloidogyne arenaria, six as M. javanica and two as M.?incognita. Meloidogyne arenaria is the predominant species with a detection rate of 61.9%, followed by M. javanica (28.5%) and M. incognita (9.5%). The tobacco RKNs were mainly distributed in western, central, and southwestern Yunnan, particularly in the western areas. Meloidogyne arenaria and M. javanica mainly occurred in Lincang (southwestern Yunnan) and Yuxi (central Yunnan), and M. incognita only in Zhaotong (northeastern Yunnan). Phylogenetic analysis based on inferences using LSU D2-D3 sequence data revealed that all 21 populations of Meloidogyne in this study are in a monophyletic clade in relation to other RKNs.
机译：在2014-2015年期间，从云南（中国）21个地区的烤烟中采集的患病根中回收到二十一个根结线虫（RKN）种群。根据28S rDNA的D2-D3扩展域序列特征和序列特征扩增区域（SCAR）对物种进行分子诊断。 SCAR结果确定了13个种群为南方根结线虫，六个为爪哇分枝杆菌，两个为隐孢子虫。南方根结线虫（Meloidogyne arenaria）是主要物种，检出率为61.9％，其次是爪哇分枝杆菌（M. javanica）（28.5％）和隐身分枝杆菌（M. incognita）（9.5％）。烟草RKNs主要分布在云南西部，中部和西南部，特别是在西部地区。南方根结线虫和爪哇分枝杆菌主要发生在临沧（云南西南部）和玉溪（云南中部），而南方根结线虫仅发生在昭通（云南北部）。根据使用LSU D2-D3序列数据推论的系统发育分析表明，在这项研究中，所有21个根结线虫种群都与其他RKN处于单系进化分支。

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