Pathogen causing Phalaenopsis soft rot disease – 16S rDNA and virulence characterisation

Sudarsono Sudarsono; Juanita Elina; Giyanto; Dewi Sukma

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Pathogen causing Phalaenopsis soft rot disease – 16S rDNA and virulence characterisation

机译：导致蝴蝶兰软腐病的病原菌– 16S rDNA和毒力鉴定

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The pathogen causing Phalaenopsis soft rot disease and developed detached leaf inoculation methods were identified. Based on its 16S rDNA sequences, the pathogen causing soft rot disease in Phalaenopsis was Erwinia chrysanthemi/Dickeya chrysanthemi. Both virulent and avirulent strains were revealed. The detached leaf inoculation assay for E. chrysanthemi/D. chrysanthemi resistance evaluation included wounding and inoculating the detached leaf with 108 CFU/ml of bacteria. Soft rot disease symptoms in the inoculated detached leaf were measurable at 20 h after inoculation. The detached leaf assay was applicable for evaluating Phalaenopsis germplasm and progeny resistance in Phalaenopsis breeding programs.

机译：确定了引起蝴蝶兰软腐病的病原体，并开发了分离的叶片接种方法。根据蝴蝶兰的16S rDNA序列，导致蝴蝶兰软腐病的病原体为菊花欧文氏菌/菊花迪卡氏菌。均显示出强毒株和无毒株。菊花E. chrysanthemi / D的离体接种法。菊花的抗药性评估包括用108 CFU / ml的细菌对伤口和伤口进行接种。接种后20 h可以测量接种的离体叶片中的软腐病症状。叶片分离试验可用于评估蝴蝶兰育种程序中的蝴蝶兰种质和后代抗性。

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《Plant Protection Science》 |2018年第1期|共8页
作者
Sudarsono Sudarsono; Juanita Elina; Giyanto; Dewi Sukma;
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中图分类植物保护;
关键词
Erwinia / Dickeya chrysanthemimoth orchidsdetached leaf inoculation assay;

机译：欧文氏菌/菊花Dickeya菊花离体叶接种试验;

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专利

1. Pathogen Causing Phalaenopsis Soft Rot Disease-16S rDNA and Virulence Characterisation [J] . Sudarsono Sudarsono, Elina Juanita, Giyanto, Plant Protection Science . 2018,第1期

机译：病原体导致鳞片肌软腐病-16s rdna和毒力表征
2. Draft Genome Sequences of Dickeya sp. Isolates B16 (NIB Z 2098) and S1 (NIB Z 2099) Causing Soft Rot of Phalaenopsis Orchids [J] . ?pela Ali?, Tina Nagli?, Pablo Llop, Genome Announcements . 2015,第5期

机译：Dickeya sp。的基因组序列草图。隔离导致蝴蝶兰软腐的B16（NIB Z 2098）和S1（NIB Z 2099）
3. Complete Genome Sequence of Dickeya dianthicola ME23, a Pathogen Causing Blackleg and Soft Rot Diseases of Potato [J] . Xing Ma, Nicole T. Perna, Jeremy D. Glasner, Genome Announcements . 2019,第7期

机译：Dickeya dianthicola ME23的完整基因组序列，该病原引起马铃薯的黑腿病和软腐病
4. Isolation and Screening of Endophytic Antagonistic Bacteria to Camellia Soft Rot Disease and 16s rDNA Sequence Analysis [C] . ZHOU Guo-ying, LI Xue-jiao, BU Ting-ting ICAMCS 2011;International conference on advanced materials and computer science . 2012

机译：茶花性软腐病内生拮抗细菌的分离筛选及16s rDNA序列分析
5. Exploring the virulence strategy of the soft-rot plant pathogen Pectobacterium carotovorum. [D] . Mole, Beth Marie. 2010

机译：探索软腐病病原菌腐食杆菌的毒性策略。
6. Draft Genome Sequences of Dickeya sp. Isolates B16 (NIB Z 2098) and S1 (NIB Z 2099) Causing Soft Rot of Phalaenopsis Orchids [O] . Špela Alič, Tina Naglič, Pablo Llop, 2015

机译：Dickeya sp。的基因组序列草图。隔离导致蝴蝶兰软腐的B16（NIB Z 2098）和S1（NIB Z 2099）
7. Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa [O] . Moleleki, Lucy N., Onkendi, Edward Makori, Mongae, Aobakwe Oratile, 2013

机译：在南非表征导致黑腿病和软腐病的pectobacterium wasabiae

Pathogen causing Phalaenopsis soft rot disease – 16S rDNA and virulence characterisation

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