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Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR

机译:快速检测椰子灵芝病并通过免疫测定和PCR评估各种控制措施的抑制作用

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Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymerase chain reaction (PCR) tests, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 167 bp in size, was used. Apparently healthy palms in two coconut gardens were tested for Ganoderma disease by ELISA test using basidiocarp protein antiserum. Field trials were laid out in these early-diagnosed palms for the management of the disease. Based on the ELISA results, Pseudomonas fluorescens + Trichoderma viride with chitin amended treatments arrested the multiplication of the pathogen and within 6 months showed an optical density (OD) below the level of infected plants. Integrated Disease Management (IDM) and fungicide tridemorph treated palms showed OD values below infection level within 7 months, and T. harzianum and P. fluorescens + T. viride treated palms showed OD values below infection level in 8 months.Keywords:Ganoderma; early diagnosis; PCR; ELISA; integrated disease managementdownload PDF Impact factor (Web of Science – Thomson Reuters) 2016: 0.742
机译:分子和免疫学方法被用于检测椰子的灵芝病。使用针对灵芝基础果皮蛋白的多克隆抗体(PAb)。对于聚合酶链反应(PCR)测试,使用了从灵芝核糖体DNA基因的内部转录间隔区1(ITS 1)产生的引物,该引物产生了167 bp的PCR产物。显然,在两个椰子园中,健康的棕榈树通过使用芥子蛋白抗血清的ELISA试验检测了灵芝病。在这些早期诊断的手掌中进行了现场试验以控制该病。根据ELISA结果,荧光假单胞菌+绿色木霉与几丁质的改良处理阻止了病原体的繁殖,并在6个月内显示出低于感染植物水平的光密度(OD)。综合疾病管理(IDM)和杀真菌剂处理过的棕榈树在7个月内OD值低于感染水平,而哈茨木霉和荧光假单胞菌+里夫螺旋藻处理过的棕榈在8个月内OD值低于感染水平。早期诊断; PCR; ELISA;疾病综合管理下载PDF影响因子(科学网-汤森路透)2016年:0.742

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