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First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic – Short Communication

机译：捷克共和国生产的胡椒种子中的胡椒轻度斑驳病毒的首次报道–简讯

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Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for Tobacco mosaic virus (TMV) but positive reactions with an antiserum to Pepper mild mottle virus (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype P1.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed.Keywords:Pepper mild mottle virus; characterisation; pathotype P1.2; Capsicum annuum; ELISA; electron microscopy; seed transmission; virus deactivation; sodium hydroxidedownload PDF Impact factor (Web of Science – Thomson Reuters) 2016: 0.742
机译：在胡椒，cv植物上观察到病毒感染的症状。 OL 228，是从2002年在温室中的捷克商品种子中长出来的。被感染的植物在叶子上显示出花叶或斑驳，在果实上显示出坏死的凹陷。通过电子显微镜发现约300 nm的直杆状病毒颗粒，表明感染了烟草花叶病毒。 ELISA对烟草花叶病毒（TMV）产生阴性反应，但对胡椒轻斑驳病毒（PMMoV）产生抗血清阳性反应。在使用具有L1，L2，L3和L4烟草花叶病毒抗性基因的辣椒品种进行生物学鉴定时，发现捷克分离株PMMoV属于P1.2型。这是PMMoV在捷克共和国的第一份报告。然而，由于一项调查未发现胡椒主要产区的其他感染，其分布仍可能受到限制。随着PMMoV随受感染种子传播，已测试并证实了其被NaOH化学灭活的可能性。表征原型P1.2;辣椒ELISA;电子显微镜;种子传播;病毒灭活;氢氧化钠下载PDF影响因子（科学网-汤森路透）2016：0.742

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