Nucleosome Presence at AML-1 Binding Sites Inversely Correlates with Ly49 Expression: Revelations from an Informatics Analysis of Nucleosomes and Immune Cell Transcription Factors

Andrew Wight; Doo Yang; Ilya Ioshikhes; Andrew P. Makrigiannis

首页> 外文期刊>PLoS Computational Biology >Nucleosome Presence at AML-1 Binding Sites Inversely Correlates with Ly49 Expression: Revelations from an Informatics Analysis of Nucleosomes and Immune Cell Transcription Factors

【24h】

Nucleosome Presence at AML-1 Binding Sites Inversely Correlates with Ly49 Expression: Revelations from an Informatics Analysis of Nucleosomes and Immune Cell Transcription Factors

机译：AML-1结合位点的核小体存在与Ly49表达成反比：从核小体和免疫细胞转录因子的信息学分析中获得的启示

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Author Summary The nucleosome-a large protein complex with DNA wound around it-is the fundamental unit of genomic organization in the eukaryotic cell. More than just a DNA organizer, however, nucleosomes may control gene expression by interfering with the cell's ability to access the wound-up DNA, as shown by recent research. In this report, we demonstrate a computational method for predicting which elements of the genome are sensitive to regulation by nucleosomes. As a proof-of-concept, we identify AML-1a binding sites-important sequences in DNA regulation-as being specifically nucleosome sensitive. We then show that AML-1a sites are specifically depleted of nucleosomes when a gene is expressed, indicating the ability for nucleosomes to suppress the expression of that gene. This finding confirms that nucleosomes are likely involved in genome regulation, and provides a method for predicting which areas of the genome are probably affected most by nucleosomes. This paper also highlights the usefulness of the Ly49 gene family in testing computer-derived genomic predictions, and is of interest to anyone studying how gene expression is regulated from cell to cell.

机译：作者总结核小体是一种大型蛋白质复合物，周围缠绕着DNA，是真核细胞基因组组织的基本单位。然而，正如最近的研究表明的那样，核小体不仅可以干扰DNA的组织者，还可以通过干扰细胞进入伤口的DNA的能力来控制基因的表达。在这份报告中，我们展示了一种预测基因组哪些元素对核小体调控敏感的计算方法。作为概念验证，我们确定AML-1a结合位点-DNA调控中的重要序列-是特异性核小体敏感的。然后，我们表明当一个基因被表达时，AML-1a位点被特异性地清除了核小体，表明核小体抑制该基因表达的能力。这一发现证实了核小体可能参与了基因组的调控，并提供了一种预测基因组哪些区域可能最受核小体影响的方法。本文还强调了Ly49基因家族在测试计算机衍生的基因组预测中的有用性，并且任何研究基因表达如何在细胞之间被调节的人都感兴趣。

著录项

来源
《PLoS Computational Biology》 |2016年第4期|共19页
作者
Andrew Wight; Doo Yang; Ilya Ioshikhes; Andrew P. Makrigiannis;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词
NucleosomesTranscription factorsNucleosome mappingGene expressionDNA transcriptionGene regulationNK cellsMammalian genomics;

机译：核小体转录因子核小体作图基因表达DNA转录基因调控NK细胞哺乳动物基因组学;

相似文献

外文文献
中文文献
专利

1. Mapping psoralen cross-links at the nucleotide levelin mammalian cells:suppression of cross-linking at transcription factor-or nucleosome-binding sites [J] . Jun-ichiro Komura, Hironobu Ikehata, Yoshio Hosoi, Biochemistry . 2001,第13期

机译：在哺乳动物细胞的核苷酸水平上定位补骨脂素交联：抑制转录因子或核小体结合位点的交联
2. Ceres: software for the integrated analysis of transcription factor binding sites and nucleosome positions in Saccharomyces cerevisiae [J] . Morris, Robert T., O'Connor, Timothy R., Wyrick, John J. Bioinformatics . 2010,第2期

机译：Ceres：用于对酿酒酵母中转录因子结合位点和核小体位置进行综合分析的软件
3. Ceres: software for the integrated analysis of transcription factor binding sites and nucleosome positions in Saccharomycesn cerevisiae [J] . John J. Wyrick Bioinformatics . 2010,第2期

机译：Ceres：用于对酿酒酵母中转录因子结合位点和核小体位置进行综合分析的软件
4. Dynamic Nucleosome Positioning around Functional Transcription Factor Binding Sites in the Promoters of Inducible NF-kappaB Target Genes [C] . Nie Yumin, Sun Xiao 5th International Conference on Bioinformatics and Biomedical Engineering . 2011

机译：动态核小体定位的功能性转录因子结合位点在诱导型NF-κB靶基因的启动子中
5. Transcription factors constitutively bind to nucleosome-free regions of the gadd45 gene during basal and induced expression. [D] . Graunke, Dawn Marie. 1999

机译：在基础和诱导表达过程中，转录因子可与gadd45基因的无核小体区域组成性结合。
6. Nucleosome Presence at AML-1 Binding Sites Inversely Correlates with Ly49 Expression: Revelations from an Informatics Analysis of Nucleosomes and Immune Cell Transcription Factors [O] . Andrew Wight, Doo Yang, Ilya Ioshikhes, 2016

机译：AML-1结合位点的核小体存在与Ly49表达成反比：从核小体和免疫细胞转录因子的信息学分析中获得的启示
7. Nucleosome Presence at AML-1 Binding Sites Inversely Correlates with Ly49 Expression: Revelations from an Informatics Analysis of Nucleosomes and Immune Cell Transcription Factors. [O] . Andrew Wight, Doo Yang, Ilya Ioshikhes, 2016

机译：amL-1结合位点的核小体存在与Ly49表达反向相关：来自核小体和免疫细胞转录因子的信息学分析的启示。
8. Chromatin Regulation of Estrogen-Mediated Transcription in Breast Cancer: Rules for Binding Sites in Nucleosomes and Modified Histones that Enhance ER Binding [R] . Chrivia, J. C. 2005

机译：染色质调节乳腺癌中雌激素介导的转录：核小体中结合位点的规则和增强ER结合的修饰组蛋白

Nucleosome Presence at AML-1 Binding Sites Inversely Correlates with Ly49 Expression: Revelations from an Informatics Analysis of Nucleosomes and Immune Cell Transcription Factors

摘要

著录项

相似文献

相关主题

期刊订阅