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首页> 外文期刊>PLoS Genetics >Escherichia coli MazF Leads to the Simultaneous Selective Synthesis of Both “Death Proteins” and “Survival Proteins”
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Escherichia coli MazF Leads to the Simultaneous Selective Synthesis of Both “Death Proteins” and “Survival Proteins”

机译：大肠杆菌MazF导致“死亡蛋白”和“生存蛋白”的同时选择性合成

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摘要

The Escherichia coli mazEF module is one of the most thoroughly studied toxin–antitoxin systems. mazF encodes a stable toxin, MazF, and mazE encodes a labile antitoxin, MazE, which prevents the lethal effect of MazF. MazF is an endoribonuclease that leads to the inhibition of protein synthesis by cleaving mRNAs at ACA sequences. Here, using 2D-gels, we show that in E. coli, although MazF induction leads to the inhibition of the synthesis of most proteins, the synthesis of an exclusive group of proteins, mostly smaller than about 20 kDa, is still permitted. We identified some of those small proteins by mass spectrometry. By deleting the genes encoding those proteins from the E. coli chromosome, we showed that they were required for the death of most of the cellular population. Under the same experimental conditions, which induce mazEF-mediated cell death, other such proteins were found to be required for the survival of a small sub-population of cells. Thus, MazF appears to be a regulator that induces downstream pathways leading to death of most of the population and the continued survival of a small sub-population, which will likely become the nucleus of a new population when growth conditions become less stressful.
机译：大肠杆菌mazEF模块是研究最深入的毒素-抗毒素系统之一。 mazF编码稳定的毒素MazF，而mazE编码不稳定的抗毒素MazE，可防止MazF的致命作用。 MazF是一种内切核糖核酸酶，可通过在ACA序列处切割mRNA来抑制蛋白质合成。在这里，我们使用2D凝胶显示，在大肠杆菌中，尽管MazF诱导导致大多数蛋白质合成的抑制，但仍允许合成唯一的蛋白质组（大多数小于20 kDa）。我们通过质谱鉴定了一些小蛋白质。通过从大肠杆菌染色体中删除编码这些蛋白质的基因，我们表明它们是大多数细胞群体死亡所必需的。在诱导mazEF介导的细胞死亡的相同实验条件下，发现其他此类蛋白质对于一小部分亚细胞的存活是必需的。因此，MazF似乎是一种调节剂，可诱导导致大多数人口死亡和小部分亚群持续存活的下游途径，当生长条件的压力降低时，这些亚群可能会成为新种群的核心。

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