...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>PLoS Genetics >Innovation of a Regulatory Mechanism Modulating Semi-determinate Stem Growth through Artificial Selection in Soybean
【24h】

Innovation of a Regulatory Mechanism Modulating Semi-determinate Stem Growth through Artificial Selection in Soybean

机译：通过人工选择调节半确定茎生长的调控机制的创新

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

It has been demonstrated that Terminal Flowering 1 ( TFL1 ) in Arabidopsis and its functional orthologs in other plants specify indeterminate stem growth through their specific expression that represses floral identity genes in shoot apical meristems (SAMs), and that the loss-of-function mutations at these functional counterparts result in the transition of SAMs from the vegetative to reproductive state that is essential for initiation of terminal flowering and thus formation of determinate stems. However, little is known regarding how semi-determinate stems, which produce terminal racemes similar to those observed in determinate plants, are specified in any flowering plants. Here we show that semi-determinacy in soybean is modulated by transcriptional repression of Dt1 , the functional ortholog of TFL1 , in SAMs. Such repression is fulfilled by recently enabled spatiotemporal expression of Dt2 , an ancestral form of the APETALA1/FRUITFULL orthologs, which encodes a MADS-box factor directly binding to the regulatory sequence of Dt1 . In addition, Dt2 triggers co-expression of the putative SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 ( GmSOC1 ) in SAMs, where GmSOC1 interacts with Dt2, and also directly binds to the Dt1 regulatory sequence. Heterologous expression of Dt2 and Dt1 in determinate ( tfl1 ) Arabidopsis mutants enables creation of semi-determinacy, but the same forms of the two genes in the tfl1 and soc1 background produce indeterminate stems, suggesting that Dt2 and SOC1 both are essential for transcriptional repression of Dt1 . Nevertheless, the expression of Dt2 is unable to repress TFL1 in Arabidopsis, further demonstrating the evolutionary novelty of the regulatory mechanism underlying stem growth in soybean. Author Summary Similar to the “green revolution” semi-dwarf cereals, semi-determinate soybean varieties are lodging-resistant and particularly suitable for planting in high fertility and irrigated environments. Nevertheless, molecular mechanisms underlying semi-determinate stem growth have not been deciphered in any flowering plants. We demonstrate that semi-determinacy is originated from an innovation of spatiotemporal expression of an ancient MADS-box gene and consequent changes of spatiotemporal expression of its interacting genes in soybean, which occurred post-domestication of soybean and selected by breeding. The findings from this study not only provides new insights into the evolutionary novelty of molecular mechanisms regulating stem growth habit reshaped by artificial selection, but also exhibited potential application of such an innovative mechanism for molecular design of stem architecture in other crops towards enhanced adaptability and yield potential.
机译：已经证明，拟南芥中的末端开花1（TFL1）及其在其他植物中的功能直系同源物通过其特异性表达抑制了茎尖分生组织（SAMs）中的花同一性基因以及功能丧失的突变，从而确定了不确定的茎生长。这些功能对应物导致SAMs从营养状态到生殖状态的转变，这对于启动终生开花并因此形成确定的茎是必不可少的。但是，关于在任何开花植物中如何确定半确定茎的知之甚少，半确定茎会产生类似于在确定植物中观察到的末端消旋。在这里，我们显示大豆中的半确定性受SAMs中Dt1（TFL1的功能直系同源物）的转录阻抑调控。通过最近启用的Dt2的时空表达来实现这种抑制，Dt2是APETALA1 / FRUITFULL直系同源物的祖先形式，其编码直接结合Dt1调控序列的MADS-box因子。此外，Dt2触发SAM中过表达的1型过表达抑制因子（GmSOC1）在SAM中的共表达，其中GmSOC1与Dt2相互作用，并且还直接与Dt1调控序列结合。 Dt2和Dt1在确定的（tfl1）拟南芥突变体中的异源表达可以创建半确定性，但是在tfl1和soc1背景中两个基因的相同形式产生不确定的茎，这表明Dt2和SOC1都是转录抑制的关键。 Dt1。然而，Dt2的表达不能抑制拟南芥中的TFL1，进一步证明了大豆茎生长调控机制的进化新颖性。作者总结与“绿色革命”的半矮谷物类似，半确定性大豆品种具有抗倒伏性，特别适合在高肥力和灌溉环境下种植。然而，在任何开花植物中，尚未确定潜在的半确定茎生长的分子机制。我们证明，半确定性起源于古代MADS-box基因的时空表达的创新及其在大豆中的相互作用基因的时空表达的变化，这种变化发生在大豆驯化后并通过选育。这项研究的发现不仅为通过人工选择重塑调控茎生长习性的分子机制的进化新颖性提供了新的见解，而且还展示了这种创新机制在其他农作物茎结构分子设计中对提高适应性和产量的潜在应用。潜在。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号