...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>PLoS Genetics >Stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells
【24h】

Stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells

机译：通过单细胞转录的定量分析揭示肠球菌性信息素反应的随机性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In Enterococcus faecalis, sex pheromone-mediated transfer of antibiotic resistance plasmids can occur under unfavorable conditions, for example, when inducing pheromone concentrations are low and inhibiting pheromone concentrations are high. To better understand this paradox, we adapted fluorescence in situ hybridization chain reaction (HCR) methodology for simultaneous quantification of multiple E. faecalis transcripts at the single cell level. We present direct evidence for variability in the minimum period, maximum response level, and duration of response of individual cells to a specific inducing condition. Tracking of induction patterns of single cells temporally using a fluorescent reporter supported HCR findings. It also revealed subpopulations of rapid responders, even under low inducing pheromone concentrations where the overall response of the entire population was slow. The strong, rapid induction of small numbers of cells in cultures exposed to low pheromone concentrations is in agreement with predictions of a stochastic model of the enterococcal pheromone response. The previously documented complex regulatory circuitry controlling the pheromone response likely contributes to stochastic variation in this system. In addition to increasing our basic understanding of the biology of a horizontal gene transfer system regulated by cell-cell signaling, demonstration of the stochastic nature of the pheromone response also impacts any future efforts to develop therapeutic agents targeting the system. Quantitative single cell analysis using HCR also has great potential to elucidate important bacterial regulatory mechanisms not previously amenable to study at the single cell level, and to accelerate the pace of functional genomic studies.
机译：在粪肠球菌中，性信息素介导的抗生素抗性质粒的转移可能在不利条件下发生，例如，诱导信息素浓度低而抑制信息素浓度高时。为了更好地理解这一悖论，我们采用了荧光原位杂交链反应（HCR）方法，以便在单个细胞水平上同时定量多个粪肠球菌转录物。我们提供了在最小周期，最大响应水平和单个细胞对特定诱导条件的响应持续时间方面的变异性的直接证据。使用荧光报告基因在时间上追踪单个细胞的诱导模式支持了HCR发现。它也揭示了快速反应者的亚群，即使在诱导信息素浓度较低的情况下，整个人群的总体反应较慢。在暴露于低信息素浓度的培养物中强烈，快速地诱导少量细胞与肠球菌信息素反应随机模型的预测一致。先前记录的控制信息素反应的复杂调节电路可能会导致该系统中的随机变化。除了增加我们对细胞信号传导调控的水平基因转移系统生物学的基本理解外，信息素应答随机性的证明还影响未来开发靶向该系统的治疗剂的任何努力。使用HCR进行定量单细胞分析也具有阐明以前不适合在单细胞水平上研究的重要细菌调控机制的巨大潜力，并可以加快功能基因组学研究的步伐。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号