• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>PLoS Genetics >Mu Transposon Insertion Sites and Meiotic Recombination Events Co-Localize with Epigenetic Marks for Open Chromatin across the Maize Genome
【24h】

Mu Transposon Insertion Sites and Meiotic Recombination Events Co-Localize with Epigenetic Marks for Open Chromatin across the Maize Genome

机译：Mu转座子插入位点和减数分裂重组事件与表观遗传标记共定位于整个玉米基因组中的开放染色质

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Mu transposon system of maize is highly active, with each of the ～50–100 copies transposing on average once each generation. The approximately one dozen distinct Mu transposons contain highly similar ～215 bp terminal inverted repeats (TIRs) and generate 9-bp target site duplications (TSDs) upon insertion. Using a novel genome walking strategy that uses these conserved TIRs as primer binding sites, Mu insertion sites were amplified from Mu stocks and sequenced via 454 technology. 94% of ～965,000 reads carried Mu TIRs, demonstrating the specificity of this strategy. Among these TIRs, 21 novel Mu TIRs were discovered, revealing additional complexity of the Mu transposon system. The distribution of >40,000 non-redundant Mu insertion sites was strikingly non-uniform, such that rates increased in proportion to distance from the centromere. An identified putative Mu transposase binding consensus site does not explain this non-uniformity. An integrated genetic map containing more than 10,000 genetic markers was constructed and aligned to the sequence of the maize reference genome. Recombination rates (cM/Mb) are also strikingly non-uniform, with rates increasing in proportion to distance from the centromere. Mu insertion site frequencies are strongly correlated with recombination rates. Gene density does not fully explain the chromosomal distribution of Mu insertion and recombination sites, because pronounced preferences for the distal portion of chromosome are still observed even after accounting for gene density. The similarity of the distributions of Mu insertions and meiotic recombination sites suggests that common features, such as chromatin structure, are involved in site selection for both Mu insertion and meiotic recombination. The finding that Mu insertions and meiotic recombination sites both concentrate in genomic regions marked with epigenetic marks of open chromatin provides support for the hypothesis that open chromatin enhances rates of both Mu insertion and meiotic recombination.
机译：玉米的Mu转座子系统非常活跃，〜50-100个拷贝中的每一个平均每世代转座一次。大约十二个不同的Mu转座子包含高度相似的〜215 bp末端反向重复序列（TIR），并在插入时产生9 bp的靶位点重复（TSD）。使用将这些保守的TIR用作引物结合位点的新型基因组步移策略，从Mu储备物中扩增了Mu插入位点，并通过454技术进行了测序。 〜96.5万次读取中有94％带有Mu TIR，证明了该策略的特异性。在这些TIR中，发现了21种新型Mu TIR，这揭示了Mu转座子系统的其他复杂性。大于40,000个非冗余Mu插入位点的分布非常不均匀，因此比率与着丝粒的距离成正比。确定的推定的Mu转座酶结合共有位点不能解释这种不均匀性。构建了包含10,000多个遗传标记的整合遗传图谱，并将其与玉米参考基因组的序列进行比对。重组率（cM / Mb）也非常不均匀，并且与离着丝粒的距离成比例地增加。 Mu插入位点频率与重组率紧密相关。基因密度不能完全解释Mu插入和重组位点的染色体分布，因为即使考虑了基因密度，仍然可以观察到对染色体末端的明显偏好。 Mu插入和减数分裂重组位点分布的相似性表明，Mu插入和减数分裂重组的位点选择均涉及共同特征，例如染色质结构。 Mu插入和减数分裂重组位点都集中在以开放染色质的表观遗传标记标记的基因组区域中的发现，为以下假设提供了支持：开放染色质可增强Mu插入和减数分裂重组的速率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号