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首页> 外文期刊>PLOS Neglected Tropical Diseases >Molecular Analysis of Echinostome Metacercariae from Their Second Intermediate Host Found in a Localised Geographic Region Reveals Genetic Heterogeneity and Possible Cryptic Speciation
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Molecular Analysis of Echinostome Metacercariae from Their Second Intermediate Host Found in a Localised Geographic Region Reveals Genetic Heterogeneity and Possible Cryptic Speciation

机译：从他们的第二个中间宿主在局部地理区域中发现棘皮动物Meta虫的分子分析揭示了遗传异质性和可能的​​隐性物种。

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Echinostome metacercariae are the infective stage for humans and animals. The identification of echinostomes has been based until recently on morphology but molecular techniques using sequences of ribosomal RNA and mitochondrial DNA have indicated major clades within the group. In this study we have used the ITS2 region of ribosomal RNA and the ND1 region of mitochondrial DNA to identify metacercariae from snails collected from eight well-separated sites from an area of 4000 km2 in Lamphun Province, Thailand. The derived sequences have been compared to those collected from elsewhere and have been deposited in the nucleotide databases. There were two aims of this study; firstly, to determine the species of echinostome present in an endemic area, and secondly, to assess the intra-specific genetic diversity, as this may be informative with regard to the potential for the development of anthelmintic resistance and with regard to the spread of infection by the definitive hosts. Our results indicate that the most prevalent species are most closely related to E. revolutum, E. trivolvis, E. robustum, E. malayanum and Euparyphium albuferensis. Some sites harbour several species and within a site there could be considerable intra-species genetic diversity. There is no significant geographical structuring within this area. Although the molecular techniques used in this study allowed the assignment of the samples to clades within defined species, however, within these groupings there were significant differences indicating that cryptic speciation may have occurred. The degree of genetic diversity present would suggest the use of targeted regimes designed to minimise the selection of anthelmintic resistance. The apparent lack of geographic structuring is consistent with the transmission of the parasites by the avian hosts.
机译：棘皮动物棘尾for是人类和动物的感染期。棘皮动物的识别直到最近才基于形态学，但是使用核糖体RNA和线粒体DNA序列的分子技术已表明该组中的主要进化枝。在这项研究中，我们使用了核糖体RNA的ITS2区域和线粒体DNA的ND1区域，从泰国南奔府4000平方公里的八个分离良好的地点收集的蜗牛中鉴定出meta尾meta。已将衍生的序列与从其他地方收集的序列进行比较，并已存储在核苷酸数据库中。这项研究有两个目的：首先，确定流行区中棘皮动物的种类，其次，评估种内遗传多样性，因为这可能对驱虫药耐药性的发展以及感染的传播具有指导意义。由确定的主机。我们的结果表明，最普遍的物种与大肠埃希氏菌，三角肠埃希氏菌，鲁棒埃希氏菌，马拉雅木埃希氏菌和白粉虫（Euparyphium albuferensis）密切相关。一些地点藏有几种物种，而一个地点内可能存在相当大的种内遗传多样性。该区域内没有重要的地理结构。尽管本研究中使用的分子技术允许将样品分配给已定义物种内的进化枝，但是，在这些组内，存在显着差异，表明可能已经发生了隐性物种形成。目前遗传多样性的程度将建议使用旨在减少驱虫药抗性选择的靶向方案。明显缺乏地理结构与禽寄主传播寄生虫是一致的。

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