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Sources of Blood Meals of Sylvatic Triatoma guasayana near Zurima, Bolivia, Assayed with qPCR and 12S Cloning

机译:qPCR和12S克隆分析玻利维亚祖里马附近的Sylvatic Triatoma guasayana的血粉来源

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Background In this study we compared the utility of two molecular biology techniques, cloning of the mitochondrial 12S ribosomal RNA gene and hydrolysis probe-based qPCR, to identify blood meal sources of sylvatic Chagas disease insect vectors collected with live-bait mouse traps (also known as Noireau traps). Fourteen T. guasayana were collected from six georeferenced trap locations in the Andean highlands of the department of Chuquisaca, Bolivia.
机译:背景技术在本研究中,我们比较了两种分子生物学技术的效用,即线粒体12S核糖体RNA基因的克隆和基于水解探针的qPCR,以鉴定通过活饵捕鼠器收集的扑热性南美锥虫病昆虫载体的血粉来源(也称为作为Noireau陷阱)。从玻利维亚丘基萨卡省安第斯山脉高地的六个地理定位陷阱位置收集了十四种危地马拉锥虫。

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