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首页> 外文期刊>PLOS Neglected Tropical Diseases >Assembly of the Genome of the Disease Vector Aedes aegypti onto a Genetic Linkage Map Allows Mapping of Genes Affecting Disease Transmission
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Assembly of the Genome of the Disease Vector Aedes aegypti onto a Genetic Linkage Map Allows Mapping of Genes Affecting Disease Transmission

机译：将埃及伊蚊的基因组组装到遗传连锁图谱上，从而可以绘制影响疾病传播的基因图谱

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The mosquito Aedes aegypti transmits some of the most important human arboviruses, including dengue, yellow fever and chikungunya viruses. It has a large genome containing many repetitive sequences, which has resulted in the genome being poorly assembled — there are 4,758 scaffolds, few of which have been assigned to a chromosome. To allow the mapping of genes affecting disease transmission, we have improved the genome assembly by scoring a large number of SNPs in recombinant progeny from a cross between two strains of Ae. aegypti, and used these to generate a genetic map. This revealed a high rate of misassemblies in the current genome, where, for example, sequences from different chromosomes were found on the same scaffold. Once these were corrected, we were able to assign 60% of the genome sequence to chromosomes and approximately order the scaffolds along the chromosome. We found that there are very large regions of suppressed recombination around the centromeres, which can extend to as much as 47% of the chromosome. To illustrate the utility of this new genome assembly, we mapped a gene that makes Ae. aegypti resistant to the human parasite Brugia malayi, and generated a list of candidate genes that could be affecting the trait.
机译：蚊埃及伊蚊传播一些最重要的人类虫媒病毒，包括登革热，黄热病和基孔肯雅病毒。它具有一个包含许多重复序列的大型基因组，这导致基因组组装不良-有4,758个支架，其中很少有人分配给染色体。为了绘制影响疾病传播的基因图谱，我们通过从两个Ae菌株之间的杂交中获得重组子代中的大量SNP来改善基因组装配。 aegypti，并用它们来生成遗传图谱。这表明当前基因组中的错配率很高，例如，在同一支架上发现了来自不同染色体的序列。纠正这些错误后，我们便能够将60％的基因组序列分配给染色体，并沿染色体大致排序支架。我们发现着丝粒周围有很大的抑制重组区域，可以延伸到染色体的47％。为了说明这种新的基因组装配的实用性，我们绘制了一个可产生Ae的基因。 aegypti对人类寄生虫马来亚布鲁加（Brugia malayi）具有抗性，并产生了可能影响该性状的候选基因列表。

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