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首页> 外文期刊>PLoS One >Signaling Specificity Provided by the Arabidopsis thaliana Heterotrimeric G-Protein γ Subunits AGG1 and AGG2 Is Partially but Not Exclusively Provided through Transcriptional Regulation
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Signaling Specificity Provided by the Arabidopsis thaliana Heterotrimeric G-Protein γ Subunits AGG1 and AGG2 Is Partially but Not Exclusively Provided through Transcriptional Regulation

机译：拟南芥异三聚体G蛋白γ亚基AGG1和AGG2提供的信号特异性是通过转录调控部分而非唯一提供的

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The heterotrimeric G-protein complex in Arabidopsis thaliana consists of one α, one ß and three γ subunits. While two of the γ subunits, AGG1 and AGG2 have been shown to provide functional selectivity to the Gßγ dimer in Arabidopsis, it is unclear if such selectivity is embedded in their molecular structures or conferred by the different expression patterns observed in both subunits. In order to study the molecular basis for such selectivity we tested genetic complementation of AGG1- and AGG2 driven by the respectively swapped gene promoters. When expressed in the same tissues as AGG1, AGG2 rescues some agg1 mutant phenotypes such as the hypersensitivity to Fusarium oxysporum and D-mannitol as well as the altered levels of lateral roots, but does not rescue the early flowering phenotype. Similarly, AGG1 when expressed in the same tissues as AGG2 rescues the osmotic stress and lateral-root phenotypes observed in agg2 mutants but failed to rescue the heat-stress induction of flowering. The fact that AGG1 and AGG2 are functionally interchangeable in some pathways implies that, at least for those pathways, signaling specificity resides in the distinctive spatiotemporal expression patterns exhibited by each γ subunit. On the other hand, the lack of complementation for some phenotypes indicates that there are pathways in which signaling specificity is provided by differences in the primary AGG1 and AGG2 amino acid sequences.
机译：拟南芥中的异三聚体G蛋白复合物由一个α，一个ß和三个γ亚基组成。虽然已经显示了两个γ亚基AGG1和AGG2对拟南芥中的Gßγ二聚体提供功能选择性，但尚不清楚这种选择性是嵌入其分子结构中还是由两个亚基中观察到的不同表达模式所赋予。为了研究这种选择性的分子基础，我们测试了分别交换的基因启动子驱动的AGG1和AGG2的遗传互补。当在与AGG1相同的组织中表达时，AGG2可以挽救一些agg1突变表型，例如对尖孢镰刀菌和D-甘露醇的超敏性以及侧根水平的改变，但不能挽救早期的开花表型。同样，当在与AGG2相同的组织中表达AGG1时，它可以挽救agg2突变体中观察到的渗透胁迫和侧根表型，但不能挽救开花的热应激诱导。 AGG1和AGG2在某些途径上在功能上可互换的事实意味着，至少对于那些途径，信号传导特异性存在于每个γ亚基表现出的独特时空表达模式中。另一方面，某些表型缺乏互补性表明存在通过主要AGG1和AGG2氨基酸序列差异提供信号传导特异性的途径。

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