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首页> 外文期刊>PLoS One >Genomic Regions Associated with Root Traits under Drought Stress in Tropical Maize (Zea mays L.)
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Genomic Regions Associated with Root Traits under Drought Stress in Tropical Maize (Zea mays L.)

机译：热带玉米（Zea mays L.）干旱胁迫下与根系性状相关的基因组区域

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An association mapping panel, named as CIMMYT Asia association mapping (CAAM) panel, involving 396 diverse tropical maize lines were phenotyped for various structural and functional traits of roots under drought and well-watered conditions. The experiment was conducted during Kharif (summer-rainy) season of 2012 and 2013 in root phenotyping facility at CIMMYT-Hyderabad, India. The CAAM panel was genotyped to generate 955, 690 SNPs through GBS v2.7 using Illumina Hi-seq 2000/2500 at Institute for Genomic Diversity, Cornell University, Ithaca, NY, USA. GWAS analysis was carried out using 331,390 SNPs filtered from the entire set of SNPs revealed a total of 50 and 67 SNPs significantly associated for root functional (transpiration efficiency, flowering period water use) and structural traits (rooting depth, root dry weight, root length, root volume, root surface area and root length density), respectively. In addition to this, 37 SNPs were identified for grain yield and shoot biomass under well-watered and drought stress. Though many SNPs were found to have significant association with the traits under study, SNPs that were common for more than one trait were discussed in detail. A total 18 SNPs were found to have common association with more than one trait, out of which 12 SNPs were found within or near the various gene functional regions. In this study we attempted to identify the trait specific maize lines based on the presence of favorable alleles for the SNPs associated with multiple traits. Two SNPs S3_128533512 and S7_151238865 were associated with transpiration efficiency, shoot biomass and grain yield under well-watered condition. Based on favorable allele for these SNPs seven inbred lines were identified. Similarly, four lines were identified for transpiration efficiency and shoot biomass under drought stress based on the presence of favorable allele for the common SNPs S1_211520521, S2_20017716, S3_57210184 and S7_130878458 and three lines were identified for flowering period water-use, transpiration efficiency, root dry weight and root volume based on the presence of favorable allele for the common SNPs S3_162065732 and S3_225760139.
机译：结合图谱命名为CIMMYT亚洲关联图谱（CAAM）面板，对396种热带玉米品系进行表型分析，确定干旱和水源充足条件下根的各种结构和功能性状。该实验是在2012年和2013年的Kharif（夏季多雨）季节在印度CIMMYT-Hyderabad的根表型研究中进行的。使用美国纽约州伊萨卡市康奈尔大学基因组多样性研究所的Illumina Hi-seq 2000/2500通过GBS v2.7对CAAM面板进行基因分型，以生成955、690个SNP。使用从整个SNP中过滤出的331,390个SNP进行了GWAS分析，发现共有50和67个SNP与根部功能（蒸腾效率，开花期用水）和结构性状（生根深度，根干重，根长）显着相关，根部体积，根部表面积和根部长度密度）。除此之外，在水分充足和干旱胁迫下，鉴定出37个单核苷酸多态性（SNP），用于谷物产量和枝条生物量。尽管发现许多SNP与所研究的性状具有显着关联，但仍详细讨论了不止一种性状的常见SNP。共发现18个SNP与一个以上的性状有共同的关联，其中12个SNP在各种基因功能区之内或附近。在这项研究中，我们尝试基于与多个性状相关的SNP的有利等位基因的存在来鉴定性状特异的玉米品系。在水分充足的条件下，两个SNP S3_128533512和S7_151238865与蒸腾效率，枝条生物量和籽粒产量相关。基于这些SNP的有利等位基因，鉴定了七个自交系。同样，根据常见SNP S1_211520521，S2_20017716，S3_57210184和S7_130878458的等位基因的有利等位基因，确定了干旱胁迫下的蒸腾效率和枝条生物量的四个系，开花期水分利用，蒸腾效率，根系干燥的三个系被确定。基于常见SNP S3_162065732和S3_225760139的有利等位基因的存在确定体重和根体积。

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