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首页> 外文期刊>PLOS Neglected Tropical Diseases >Trends in the Epidemiology of Pandemic and Non-pandemic Strains of Vibrio parahaemolyticus Isolated from Diarrheal Patients in Kolkata, India
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Trends in the Epidemiology of Pandemic and Non-pandemic Strains of Vibrio parahaemolyticus Isolated from Diarrheal Patients in Kolkata, India

机译：印度加尔各答腹泻患者大副溶血性弧菌大流行和非大流行毒株的流行病学趋势

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A total of 178 strains of V. parahaemolyticus isolated from 13,607 acute diarrheal patients admitted in the Infectious Diseases Hospital, Kolkata has been examined for serovar prevalence, antimicrobial susceptibility and genetic traits with reference to virulence, and clonal lineages. Clinical symptoms and stool characteristics of V. parahaemolyticus infected patients were analyzed for their specific traits. The frequency of pandemic strains was 68%, as confirmed by group-specific PCR (GS-PCR). However, the prevalence of non-pandemic strains was comparatively low (32%). Serovars O3:K6 (19.7%), O1:K25 (18.5%), O1:KUT (11.2%) were more commonly found and other serovars such as O3:KUT (6.7%), O4:K8 (6.7%), and O2:K3 (4.5%) were newly detected in this region. The virulence gene tdh was most frequently detected in GS-PCR positive strains. There was no association between strain features and stool characteristics or clinical outcomes with reference to serovar, pandemicon-pandemic or virulence profiles. Ampicillin and streptomycin resistance was constant throughout the study period and the MIC of ampicillin among selected strains ranged from 24 to >256 μg/ml. Susceptibility of these strains to ampicillin increased several fold in the presence of carbonyl cyanide-m-chlorophenyldrazone. The newly reported ESBL encoding gene from VPA0477 was found in all the strains, including the susceptible ones for ampicillin. However, none of the strains exhibited the β-lactamase as a phenotypic marker. In the analysis of pulsed-field gel electrophoresis (PFGE), the pandemic strains formed two different clades, with one containing the newly emerged pandemic strains in this region.
机译：从加尔各答传染病医院住院的13607例急性腹泻患者中分离出总共178株副溶血性弧菌，并根据毒力和克隆谱系检查了它们的血清型流行率，抗药性和遗传特征。分析副溶血性弧菌感染患者的临床症状和粪便特征，了解其特定特征。大流行株的频率为68％，这已通过组特异性PCR（GS-PCR）确认。但是，非大流行毒株的患病率相对较低（32％）。血清O3：K6（19.7％），O1：K25（18.5％），O1：KUT（11.2％）更为常见，其他血清型如O3：KUT（6.7％），O4：K8（6.7％）和在该区域新发现了O2：K3（4.5％）。在GS-PCR阳性菌株中最常检测到毒力基因tdh。血清型，大流行/非大流行或毒力谱系与菌株特征和粪便特征或临床结果之间没有关联。在整个研究期间，氨苄青霉素和链霉素的耐药性均保持恒定，所选菌株中氨苄青霉素的MIC范围为24至> 256μg/ ml。在羰基氰化物-间氯苯基in存在下，这些菌株对氨苄青霉素的敏感性增加了几倍。在所有菌株中都发现了新近报道的来自VPA0477的ESBL编码基因，包括对氨苄青霉素敏感的菌株。但是，没有菌株表现出β-内酰胺酶作为表型标记。在脉冲场凝胶电泳（PFGE）分析中，大流行株形成了两个不同的进化枝，其中一个包含该区域中新出现的大流行株。

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