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首页> 外文期刊>Poultry Science Journal >Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers
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Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers

机译：利用微卫星标记评估日语和英语白鹌鹑种群的遗传多样性

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The Japanese and English White quails are widespread strains and belongs to the Galliformes order, Phasianidae family, Coturnix genus and Japonica species. These birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. In the current study, the genetic variation of Japanese and English White quail populations were studied. Frequency of polymorphic loci, polymorphic information content, heterozygosity, Shannon's information index, number of observed and effective alleles were assessed using 4 microsatellite markers with high polymorphic information content value (GUJ0034, GUJ0049, GUJ0080 and GUJ0097). The Blood samples were collected randomly from 50 Japanese quails and 50 English White quails rearing in the research center of Gorgan University of Agricultural Sciences and Natural Resources. The genomic DNA was extracted using DIAtom DNA Prep 100 kit, and its quality and quantity were determined using electrophoresis gel and spectrophotometery methods. The PCR reactions were successfully performed with four microsatellite markers. The results based on the chi-square and likelihood ratio tests showed a significant deviation from Hardy-Weinberg equilibrium. The means of genetic diversity parameters such as number of effective alleles, the number of observed alleles, the expected and observed heterozygosity, Shannon's information index and PIC in quail populations were 4.78±0.37, 7.50±0.57, 0.79±0.02, 0.60±0.16, 1.73±0.05 and 0.76±0.02 respectively. The results of the current study showed that the investigated quail populations have a relatively high genetic diversity with respect to the applied microsatellite markers and confirmed prior study’s findings on the ability of microsatellite markers in investigating genetic diversity.
机译：日本和英国的白鹌鹑是广泛分布的品系，属于鸡形目，as科，Coturnix属和粳稻。这些鸟类可能已经很好地适应了艰苦的条件和对疾病的抵抗力，因为它已作为一种农业物种获得了经济重要性。在本研究中，研究了日本和英国白鹌鹑种群的遗传变异。使用4个具有高多态信息含量值（GUJ0034，GUJ0049，GUJ0080和GUJ0097）的微卫星标记，评估了多态位点的频率，多态信息含量，杂合性，香农信息指数，观察到的有效等位基因数量。从Gorgan农业科学与自然资源研究中心饲养的50只日本鹌鹑和50只英国白鹌鹑中随机采集血样。使用DIAtom DNA Prep 100试剂盒提取基因组DNA，并使用电泳凝胶和分光光度法确定其质量和数量。用四个微卫星标记成功进行了PCR反应。基于卡方和似然比检验的结果表明，与Hardy-Weinberg平衡存在显着偏差。遗传多样性参数的方法，例如有效等位基因数量，观察到的等位基因数量，预期和观察到的杂合性，香农信息指数和鹌鹑种群中的PIC，分别为4.78±0.37、7.50±0.57、0.79±0.02、0.60±0.16，分别为1.73±0.05和0.76±0.02。当前研究的结果表明，就所应用的微卫星标记而言，被调查的鹌鹑种群具有相对较高的遗传多样性，并证实了先前研究中有关微卫星标记研究遗传多样性能力的研究结果。

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