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NGS IDENTIFICATION OF FUNGI POTENTIALLY IMPLICATED IN THE PRODUCTION OF AGARWOOD FROM AQUILARIA SPP. TREES

机译:NGS鉴定潜在地暗示了从Aquilaria SPP生产沉香木中的真菌。树

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Aquilaria is a tree species belonging to the Thymeleaceae family. When Aquilaria sp. is injured, it can produce agarwood. Agarwood is characterized by a darker wood colour than the healthy one and by a strong perfume that is much esteemed by perfumers and some oriental religious communities.The production of agarwood is presumed to depend on environmental factors, among them fungi.The aim of this work is to obtain an overview of fungi present in Aquilaria sp. from different countries. Aquilaria sp. is endemic to South East Asia including notably Cambodia, Laos and Thailand, where it is cultivated to produce agarwood. In French Guiana, farmers would like to locally produce agarwood in their field. That’s why we wonder if fungal communities naturally present in French Guiana present characteristics making it compatible with the induction of agarwood.In this study, NGS was used to characterize fungal communities associated with agarwood: 693,961 sequences that cover ITS2 estimated about 250bp have been obtained. These sequences have been grouped into 535 OTUs, displaying 100% identity. In this study, 87% were Ascomycetes and 10.5% were Basidiomycetes.These results show also differences in fungal communities between aboveground and belowground parts of the tree. Likewise, differences between countries within fungal communities were also observed.
机译:沉香是属于百里香科的一种树种。当沉香。受伤,会产生沉香。沉香的特点是比健康的木色更深的颜色,以及浓烈的香气,香精被调香师和一些东方宗教团体所推崇。据推测,沉香的生产取决于环境因素,其中包括真菌。是为了获得沉香菌中存在的真菌的概述。来自不同国家沉香是东南亚的特有种,主要种植柬埔寨,老挝和泰国,在那里种植沉香木。在法属圭亚那,农民想在自己的田里当地生产沉香木。因此,我们想知道法属圭亚那天然存在的真菌群落是否具有与沉香诱导有关的特性。在这项研究中,NGS用于鉴定与沉香相关的真菌群落:已获得693,961个覆盖ITS2的序列,估计约250bp。这些序列已分组为535个OTU,显示出100%的同一性。在这项研究中,子囊菌为87%,担子菌为10.5%,这些结果还表明树的地上部分和地下部分之间的真菌群落存在差异。同样,也观察到真菌群落内国家之间的差异。

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