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首页> 外文期刊>Revista Brasileira de Ciência Avícola >Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil
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Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil

机译:从巴西家禽来源分离的肠炎沙门氏菌血清型的抗药性和分子表征

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Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study was to determine the antimicrobial resistance patterns for Salmonella serotypes isolated from poultry sources in Brazil and to detect virulence-associated genes and verify their association with specific serotypes. A total of 163 strains of Salmonella enterica isolated from poultry sources in Southern Brazil were selected, and each belonged to one of 11 different serotypes. They were tested against ten antibiotics and examined for the presence of 26 virulence-associated genes by PCR. S . Typhimurium, S . Bredeney, S . Schwarzengrund and S . Tennessee showed the highest overall resistance rates. Approximately 18% of Salmonella strains were classified as multidrug-resistant strains. Our results indicate associations between antimicrobial resistance and specific serotypes. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the serotype. Eight genes are positively or negatively associated with at least one serotype. The observed associations between antimicrobial resistance and specific serotypes are useful in developing specific control and treatment measures for each serotype. Despite the virulence genes being evenly distributed among the serotypes, some of these genes are associated with specific serotypes, and sefA, sopE and lpfA were selected as possible markers of Salmonella serotypes.
机译:沙门氏菌仍然是全世界食源性疾病最重要的媒介之一。沙门氏菌的重要性。在公共卫生领域的应用与它们广泛的抗菌素耐药性及其在人和动物宿主中的致病性和毒力有关。这项研究的目的是确定从巴西家禽来源分离的沙门氏菌血清型的抗药性模式,并检测与毒力相关的基因,并验证其与特定血清型的关联。总共从巴西南部的家禽源中分离出了163株肠炎沙门氏菌菌株,每一种都属于11种不同血清型之一。他们针对十种抗生素进行了测试,并通过PCR检查了26种与毒力相关的基因的存在。 。鼠伤寒沙门氏菌。布雷迪尼,S。 Schwarzengrund和S。田纳西州的总体抵抗率最高。约18%的沙门氏菌菌株被归类为耐多药菌株。我们的结果表明抗菌素耐药性与特定血清型之间的关联。不论血清型如何,大多数研究的基因呈现高频率和规则分布。八个基因与至少一种血清型正相关或负相关。所观察到的抗菌素耐药性与特定血清型之间的关联对于开发针对每种血清型的特定控制和治疗措施很有用。尽管毒力基因在血清型之间平均分布,但其中一些基因与特定血清型相关,并且选择了sefA,sopE和lpfA作为沙门氏菌血清型的标记。

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