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首页> 外文期刊>Revista Brasileira de Ciência Avícola >Molecular Authentication of Meats from Three Terrestrial Birds Based on Pcr-Rflp Analysis of the Mitochondrial 12S rRNA Gene
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Molecular Authentication of Meats from Three Terrestrial Birds Based on Pcr-Rflp Analysis of the Mitochondrial 12S rRNA Gene

机译：基于线粒体12S rRNA基因的Pcr-Rflp分析，对三只陆生鸟类的肉进行分子鉴定

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In this study, a method utilizing PCR-restriction fragment length polymorphism (PCR-RFLP) of a mitochondrial gene was developed for the identification of chicken ( Gallus gallus ), quail ( Coturnix coturnix ), and common pigeon ( Columba livia ) meat. PCR products of ~440 bp were obtained from the 12S rRNA gene of these three birds using a pair of universal primers. The three terrestrial birds can be distinguished using one restriction endonuclease, Alu I, which was selected based on species-specific variations in the mt 12S rRNA gene sequence using 9 newly-obtained and 44 published chicken, quail and pigeon sequences. This method was also successfully used to identify commercial quail and pigeon meat products, which were found to be adulterated with chicken meat. Additionally, our method had relatively high sensitivity for detecting a meat mixture. Ten percent of chicken meat in the mixed quail and pigeon sample was detectable. This assay can be useful for the accurate identification of meats from terrestrial birds, avoiding mislabeling or fraudulent species substitution in meat products.
机译：在这项研究中，开发了一种利用线粒体基因的PCR限制性片段长度多态性（PCR-RFLP）来鉴定鸡肉（鸡），鹌鹑（Coturnix coturnix）和普通鸽（Columba livia）肉的方法。使用一对通用引物，从这三只鸟的12S rRNA基因中获得约440 bp的PCR产物。可以使用一种限制性核酸内切酶Alu I来区分这三只陆生鸟类，该酶是根据mt 12S rRNA基因序列中物种特异性的变化而选择的，其中使用了9种新近获得的和公开发表的44种鸡，鹌鹑和鸽子序列。该方法也成功地用于鉴定商品鹌鹑和鸽子肉产品，这些产品被掺假了鸡肉。此外，我们的方法对检测肉类混合物具有较高的灵敏度。在鹌鹑和鸽子混合样本中可检测到10％的鸡肉。该测定法可用于准确识别陆生鸟类的肉，避免肉类产品中标签错误或欺诈性物种替代。

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