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首页> 外文期刊>Proteome science >Proteome changes induced by Pyrenophora tritici-repentis ToxA in both insensitive and sensitive wheat indicate senescence-like signaling
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Proteome changes induced by Pyrenophora tritici-repentis ToxA in both insensitive and sensitive wheat indicate senescence-like signaling

机译：小麦和小麦不敏感和敏感小麦中均由Pyrenophora tritici-repentis ToxA诱导的蛋白质组变化表明衰老样信号

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Background Pyrenophora tritici-repentis is a phytopathogenic fungus which causes tan spot on wheat. Some races of P. tritici-repentis produce host-specific toxins which present symptoms of chlorosis or necrosis on susceptible wheat cultivars. One such toxin is Ptr ToxA, which enters mesophyll cells through a putative toxin-receptor and localizes with chloroplasts, ultimately causing damage and necrosis on leaves. These symptoms can occur even in the absence of the pathogen. Insensitive cultivars lack the receptor and Ptr ToxA cannot enter cells. The molecular mechanisms surrounding this plant-pathogen interaction are still largely unknown, although some details have begun to emerge. Results Using 2-D electrophoresis, fifteen protein changes were identified reproducibly in the leaf proteomes of a sensitive and an insensitive cultivar over three days after inoculation of purified Ptr ToxA. Functional analysis of the proteins indicated that senescence signals may be induced in the sensitive cultivar. In the insensitive cultivar proteins involved in some features of senescence inhibition were seen. Complementary responses at the biochemical level may be actively promoting a localized senescence-like response in sensitive wheat cultivars whilst actively inhibiting this response in insensitive cultivars. Conclusion This is the first report of a biochemical response in an insensitive cultivar in this plant-pathogen interaction. Findings support the involvement of ethylene, and the activation of complementary pathways in sensitive versus insensitive wheat cultivars responding to Ptr ToxA. The nature of the system permits using purified toxin to mimic disease, which eliminates the pathogen proteome and ensures a synchronous response in inoculated leaves.
机译：背景Pyrenophora tritici-repentis是一种致病真菌，在小麦上引起棕褐色斑点。小麦小黑麦的某些种族产生宿主特异性毒素，在易感小麦品种上出现萎黄或坏死的症状。一种这样的毒素是Ptr ToxA，它通过推定的毒素受体进入叶肉细胞并定位在叶绿体中，最终导致叶片受损和坏死。即使没有病原体，也会出现这些症状。不敏感的品种缺乏受体，Ptr ToxA无法进入细胞。尽管一些细节已经开始出现，但是围绕这种植物-病原体相互作用的分子机制仍然很大程度上未知。结果使用二维电泳，在接种纯化的Ptr ToxA后的三天内，可敏感和不敏感品种的叶片蛋白质组中可重复地鉴定出15种蛋白质变化。蛋白质的功能分析表明，可以在敏感品种中诱导衰老信号。在不敏感的变种蛋白中观察到了衰老抑制的某些特征。在生化水平上的互补反应可能在敏感的小麦品种中积极促进局部衰老样反应，而在不敏感的品种中积极抑制这种反应。结论这是关于这种植物-病原体相互作用中不敏感品种的生化反应的首次报道。研究结果支持乙烯的参与以及敏感和不敏感小麦品种对Ptr ToxA的响应中互补途径的激活。该系统的性质允许使用纯化的毒素模拟疾病，从而消除了病原体蛋白质组，并确保了接种叶片中的同步响应。

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