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Comparative proteomic analyses of Asian cotton ovules with attached fibers in the early stages of fiber elongation process

机译：亚洲棉胚珠在纤维伸长过程早期的比较蛋白质组学分析

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Background Plenty of proteomic studies were performed to characterize the allotetraploid upland cotton fiber elongation process, whereas little is known about the elongating diploid cotton fiber proteome. Methods In this study, we used a two-dimensional electrophoresis-based comparative proteomic approach to profile dynamic proteomes of diploid Asian cotton ovules with attached fibers in the early stages of fiber elongation process. One-way ANOVA and Student-Newman-Keuls test were used to find the differentially displayed protein (DDP) spots. Results A total of 55 protein spots were found having different abundance ranging from 1 to 9?days post-anthesis (DPA) in a two-day interval. These 55 DDP spots were all successfully identified using high-resolution mass spectrometric analyses. Gene ontology analyses revealed that proteoforms involved in energy/carbohydrate metabolism, redox homeostasis, and protein metabolism are the most abundant. In addition, orthologues of the 13 DDP spots were also found in differential proteome of allotetraploid elongating cotton fibers, suggesting their possible essential roles in fiber elongation process. Conclusions Our results not only revealed the dynamic proteome change of diploid Asian cotton fiber and ovule during early stages of fiber elongation process but also provided valuable resource for future studies on the molecular mechanism how the polyploidization improves the trait of fiber length.
机译：背景技术进行了大量的蛋白质组学研究来表征异源二倍体陆地棉纤维的延伸过程，而对伸长的二倍体棉纤维蛋白质组的了解很少。方法在这项研究中，我们使用了基于二维电泳的比较蛋白质组学方法，在纤维伸长过程的早期阶段，对附着有纤维的二倍体亚洲棉胚珠的动态蛋白质组进行了分析。单向方差分析和Student-Newman-Keuls测试用于发现差异显示的蛋白质（DDP）点。结果发现在花后1天到9天之间，以两天为间隔，共有55个蛋白点具有不同的丰度。使用高分辨率质谱分析已成功识别出这55个DDP点。基因本体分析表明，参与能量/碳水化合物代谢，氧化还原稳态和蛋白质代谢的蛋白形式最丰富。此外，在异源四倍体伸长的棉纤维的差异蛋白质组中也发现了13个DDP点的直向同源物，表明它们在纤维伸长过程中可能发挥重要作用。结论我们的研究结果不仅揭示了亚洲二倍体棉纤维和胚珠在纤维伸长过程早期的动态蛋白质组变化，而且为今后研究多倍化如何改善纤维长度性状的分子机理提供了宝贵的资源。

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