• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proteome science >Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum
【24h】

Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

机译：使用基于LC-MS / MS的血清蛋白质组学分析表征健康绵羊的循环脱细胞蛋白质组

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundUnlike humans, there is currently no publicly available reference mass spectrometry-based circulating acellular proteome data for sheep, limiting the analysis and interpretation of a range of physiological changes and disease states. The objective of this study was to develop a robust and comprehensive method to characterise the circulating acellular proteome in ovine serum. MethodsSerum samples from healthy sheep were subjected to shotgun proteomic analysis using nano liquid chromatography nano electrospray ionisation tandem mass spectrometry (nanoLC-nanoESI-MS/MS) on a quadrupole time-of-flight instrument (TripleTOF? 5600+, SCIEX). Proteins were identified using ProteinPilot? (SCIEX) and Mascot (Matrix Science) software based on a minimum of two unmodified highly scoring unique peptides per protein at a false discovery rate (FDR) of 1% software by searching a subset of the Universal Protein Resource Knowledgebase (UniProtKB) database ( http://www.uniprot.org ). PeptideShaker (CompOmics, VIB-UGent) searches were used to validate protein identifications from ProteinPilot? and Mascot. ResultsProteinPilot? and Mascot identified 245 and 379 protein groups (IDs), respectively, and PeptideShaker validated 133 protein IDs from the entire dataset. Since Mascot software is considered the industry standard and identified the most proteins, these were analysed using the Protein ANalysis THrough Evolutionary Relationships (PANTHER) classification tool revealing the association of 349 genes with 127 protein pathway hits. These data are available via ProteomeXchange with identifier PXD004989. ConclusionsThese results demonstrated for the first time the feasibility of characterising the ovine circulating acellular proteome using nanoLC-nanoESI-MS/MS. This peptide spectral data contributes to a protein library that can be used to identify a wide range of proteins in ovine serum.
机译：背景技术与人类不同，目前尚无可公开获得的基于参考绵羊质谱的循环脱细胞蛋白质组数据，从而限制了对一系列生理变化和疾病状态的分析和解释。这项研究的目的是开发一种强大而全面的方法来表征绵羊血清中的循环脱细胞蛋白质组。方法使用四极杆飞行时间仪器（TripleTOF？5600 +，SCIEX），使用纳米液相色谱纳米电喷雾串联质谱（nanoLC-nanoESI-MS / MS）对健康绵羊的血清样本进行shot弹枪蛋白质组分析。使用ProteinPilot？鉴定蛋白质。 （SCIEX）和Mascot（Matrix Science）软件，通过搜索通用蛋白质资源知识库（UniProtKB）数据库的子集，以每个蛋白质至少两个未修饰的高评分独特肽为基础，错误发现率（FDR）为1％。 http://www.uniprot.org）。使用PeptideShaker（CompOmics，VIB-UGent）搜索来验证ProteinPilot？和吉祥物。结果蛋白质飞行员？ Mascot和Mascot分别识别了245个和379个蛋白质组（ID），PeptideShaker验证了整个数据集中的133个蛋白质ID。由于Mascot软件被认为是行业标准并能识别最多的蛋白质，因此使用“蛋白质分析通向进化关系”（PANTHER）分类工具对这些蛋白质进行了分析，揭示了349个基因与127个蛋白质途径的关联。这些数据可通过ProteomeXchange获得，其标识符为PXD004989。结论这些结果首次证明了使用nanoLC-nanoESI-MS / MS表征绵羊循环无细胞蛋白质组的可行性。该肽谱数据有助于建立蛋白质文库，可用于鉴定绵羊血清中的多种蛋白质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号