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首页> 外文期刊>Proteome science >MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration
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MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration

机译：MS / MS文库有助于变性超滤制备的天然肽的MRM定量

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摘要

Naturally occurring native peptides provide important information about physiological states of an organism and its changes in disease conditions but protocols and methods for assessing their abundance are not well-developed. In this paper, we describe a simple procedure for the quantification of non-tryptic peptides in body fluids. The workflow includes an enrichment step followed by two-dimensional fractionation of native peptides and MS/MS data management facilitating the design and validation of LC- MRM MS assays. The added value of the workflow is demonstrated in the development of a triplex LC-MRM MS assay used for quantification of peptides potentially associated with the progression of liver disease to hepatocellular carcinoma.
机译：天然存在的天然肽提供了有关生物体生理状态及其疾病状况变化的重要信息，但评估其丰度的方案和方法尚不完善。在本文中，我们描述了一种简单的定量体液中非胰蛋白酶肽的方法。工作流程包括富集步骤，然后是天然肽的二维分级分离和MS / MS数据管理，有助于LC-MRM MS分析的设计和验证。该工作流程的附加价值在开发用于定量可能与肝脏疾病发展为肝细胞癌相关的肽的三重LC-MRM MS分析中得到了证明。

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