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首页> 外文期刊>Proteome science >Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells
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Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells

机译：高迁移率B组蛋白家族的成员在胚胎神经干细胞中动态表达

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Neural Stem Cells (NSCs) are a distinct group of cells present in the embryonic and adult mammalian central nervous system (CNS) that are able to differentiate into neurons, astrocytes and oligodendrocytes. As NSC proliferation declines with age, factors that regulate this process need to be defined. To search for NSC regulatory factors, we performed a quantitative shotgun proteomics study that revealed that members of the High Mobility Group B (HMGB) family are highly expressed in NSCs. Using a neurosphere assay, we report the differential expression of HMGB 1, 2, 3, and 4 mRNAs in proliferating NSCs isolated from various time points during embryonic development, as well as the dynamic expression of HMGB1 and B2 mRNAs and proteins in differentiating embryonic NSCs. Expression of HMGB2 underwent the most dramatic changes during the developmental ages examined; as a result, we assessed its role in NSC proliferation and differentiation. We report the predominance of small diameter HMGB2-/- neurospheres in comparison to wild-type, which correlated with increased proliferation in these smaller HMGB2-/- neurospheres. Our data suggest that HMGB2 plays a regulatory role in NSC cell proliferation and maintenance pathways.
机译：神经干细胞（NSC）是存在于胚胎和成年哺乳动物中枢神经系统（CNS）中的一组独特细胞，它们能够分化为神经元，星形胶质细胞和少突胶质细胞。随着NSC增殖随着年龄的增长而下降，需要定义调节这一过程的因素。为了搜索NSC调控因子，我们进行了定量shot弹枪蛋白质组学研究，结果表明，高机动性B组（HMGB）家族成员在NSC中高度表达。使用神经球分析，我们报道了在胚胎发育过程中从各个时间点分离的增殖NSC中HMGB 1、2、3和4 mRNA的差异表达，以及在分化胚胎NSC中HMGB1，B2 mRNA和蛋白的动态表达。 。在所研究的发育年龄中，HMGB2的表达发生了最明显的变化。结果，我们评估了其在NSC增殖和分化中的作用。我们报告了与野生型相比，小直径HMGB2-/-神经球的优势，这与这些较小的HMGB2-/-神经球的增殖增加相关。我们的数据表明，HMGB2在NSC细胞增殖和维持途径中起调节作用。

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