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首页> 外文期刊>Proteome science >Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus
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Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus

机译：利用二维差异凝胶电泳鉴定传染性胃肠炎冠状病毒感染的ST细胞中的细胞蛋白质组

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Background Transmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs, which is correlated with high morbidity and mortality in suckling piglets. Information remains limited about the comparative protein expression of host cells in response to TGEV infection. In this study, cellular protein response to TGEV infection in swine testes (ST) cells was analyzed, using the proteomic method of two-dimensional difference gel electrophoresis (2D DIGE) coupled with MALDI-TOF-TOF/MS identification. Results 33 differentially expressed protein spots, of which 23 were up-regulated and 10 were down-regulated were identified. All the protein spots were successfully identified. The identified proteins were involved in the regulation of essential processes such as cellular structure and integrity, RNA processing, protein biosynthesis and modification, vesicle transport, signal transduction, and the mitochondrial pathway. Western blot analysis was used to validate the changes of alpha tubulin, keratin 19, and prohibitin during TGEV infection. Conclusions To our knowledge, we have performed the first analysis of the proteomic changes in host cell during TGEV infection. 17 altered cellular proteins that differentially expressed in TGEV infection were identified. The present study provides protein-related information that should be useful for understanding the host cell response to TGEV infection and the underlying mechanism of TGEV replication and pathogenicity.
机译：背景传染性胃肠炎冠状病毒（TGEV）是一种肠致病性冠状病毒，可引起猪的腹泻，这与哺乳仔猪的高发病率和高死亡率有关。关于宿主细胞响应TGEV感染的比较蛋白表达的信息仍然有限。在这项研究中，使用蛋白质组学方法通过二维差异凝胶电泳（2D DIGE）结合MALDI-TOF-TOF / MS鉴定分析了猪睾丸（ST）细胞中TGEV感染的细胞蛋白反应。结果鉴定出33个差异表达的蛋白斑点，其中23个上调而10个下调。所有蛋白斑点均已成功鉴定。鉴定出的蛋白质参与基本过程的调控，例如细胞结构和完整性，RNA加工，蛋白质生物合成和修饰，囊泡运输，信号转导和线粒体途径。 Western blot分析用于验证TGEV感染期间α微管蛋白，角蛋白19和抑制素的变化。结论据我们所知，我们已经对TGEV感染过程中宿主细胞的蛋白质组学变化进行了首次分析。鉴定了在TGEV感染中差异表达的17种改变的细胞蛋白。本研究提供蛋白质相关的信息，应有助于理解宿主细胞对TGEV感染的反应以及TGEV复制和致病性的潜在机制。

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