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首页> 外文期刊>Psyche >Morphology and Ultrastructure of Brain Tissue and Fat Body from the Flesh Fly,Sarcophaga bullataParker (Diptera: Sarcophagidae), Envenomated by the Ectoparasitic WaspNasonia vitripennis(Walker) (Hymenoptera: Pteromalidae)
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Morphology and Ultrastructure of Brain Tissue and Fat Body from the Flesh Fly,Sarcophaga bullataParker (Diptera: Sarcophagidae), Envenomated by the Ectoparasitic WaspNasonia vitripennis(Walker) (Hymenoptera: Pteromalidae)

机译:肉蝇苍蝇的大脑组织和脂肪体的形态和超微结构,由寄生寄生的黄蜂(Walker)毒死(膜翅目:翼手龙科)

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This study tested the hypothesis that venom from the ectoparasitic waspNasonia vitripennistargets brain tissue and fat body from its flesh fly host,Sarcophaga bullata. By 1 h postenvenomation, some brain neurons began to show irregularities in nuclear shape, and though they were predominately euchromatic, there was evidence of heterochromatin formation. Irregularity in the nuclear envelope became more prominent by 3 h after envenomation, as did the condensation of heterochromatin. The severity of ultrastructural changes continued to increase until at least 24 h after parasitoid attack. At this point, cellular swelling and extensive heterochromatic inclusions were evident, multivesicular bodies occurred in the cytoplasm of some cells, and the rough endoplasmic reticulum was dilated in many of the cells. Immunohistochemical staining revealed significant apoptosis in neurons located in brain tissues. By contrast, there was no evidence of any morphological or ultrastructural disturbances in fat body tissues up to 24 h after envenomation, nor did any of the cells display signs of cell death.
机译:这项研究检验了一种假设,即来自外寄生性黄蜂的毒液可能来自拟南芥(Vistenennis vitripennis),其目标是来自其肉蝇寄主Sa(Sarcophaga bullata)的脑组织和脂肪体。毒死后1小时,一些脑神经元开始显示核形状不规则,尽管它们主要是常染色,但有异染色质形成的迹象。毒化后3 h,核膜的不规则性变得更加明显,异染色质的凝结也是如此。超结构变化的严重性持续增加,直到类寄生虫发作后至少24 h。在这一点上,明显的细胞肿胀和广泛的异色夹杂物,一些细胞的细胞质中出现多囊泡体,并且许多细胞中的粗面内质网被扩张。免疫组织化学染色显示位于脑组织的神经元中存在明显的细胞凋亡。相比之下,没有证据表明在毒化后24h内,脂肪体组织有任何形态或超微结构的紊乱,也没有任何细胞显示出细胞死亡的迹象。

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